增生性玻璃体视网膜病变增生膜中结缔组织生长因子与转化生长因子β受体和细胞外基质相关性的研究  

The correlation among connective tissue growth factor,transforming growth factor-β receptor,and extracellular matrix in human proliferative membranes of proliferative vitreoretinopathy

作　　者：郭长梅[1] 惠延年[1] 王雨生[1] 闫峰[1] 马吉献[1] 杜红俊[1] 

机构地区：[1]第四军医大学西京医院眼科,西安710032

出　　处：《中华眼底病杂志》2006年第3期192-195,共4页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金资助项目(39970780)

摘　　要：目的观察结缔组织生长因子(CTGF)在不同级别增生性玻璃体视网膜病变(PVR)增生膜中的表达及其与转化生长因子β受体(TGF-βR)和细胞外基质(ECM)出现的关系,探讨PVR发病过程中CTGF与TGF-βR和ECM产生的相关性。方法采用链霉亲和素-生物素复合物(SABC)免疫组织化学方法,检测玻璃体切除手术获得的43例PVR增生膜中CTGF、TGF-βR、纤维连接蛋白(FN)和型及型胶原蛋白的表达,应用Spearman相关分析判断两样本之间有无相关性。结果PVR增生膜中CTGF、TGF-βR蛋白高表达,阳性表达的细胞主要是上皮样细胞。免疫组织化学显示C级膜中二者阳性表达率分别为70.6%和76.5%,D级膜中分别为73.9%和69.6%。统计学分析结果显示,CTGF、TGF-βR各级阳性表达率与膜分级间无相关性(P>0.05)。PVR膜中FN、型和型胶原染色在实质细胞和细胞外间质均有表达;统计学分析结果显示,CTGF与TGF-βR、FN、型及型胶原的表达呈正相关(P<0.01)。结论PVR增生膜中CTGF、TGF-βR蛋白表达上调,推测CTGF的产生与TGF-βR的激活有关,CTGF加重增生膜中RPE细胞合成FN和胶原等ECM,参与了PVR增生膜的形成和发展。Objective To investigate the expression of connective tissue growth factor （CTGF）in human proliferative membranes of proliferative vitreoretinopathy （PVR）, and the relationship among CTGF,transforming growth factor-β receptor （TGF-βR）and extracellular matrix （ECM）. Methods Immunohistochemistry method of streptavidin-biotin-peroxidase complex （SABC）was used to detect the expression of CTGF, TGF-βRⅡ , fibronectin （FN）, collagen Ⅰ , and collagen Ⅱ protein in43periretinal membranes （PRM）of PVR obtained by vitrectomy,and the correlations of the expression of CTGF,TGF- βR Ⅱ and ECM were analyzed by statistics. Results CTGF and TGF-βR Ⅱ protein highly expressed in PRM of PVR and most of the CTGF-positive cells were epithelial cells. The result of immunohistochemistry showed that the positive rates of CTGF and TGF-βR Ⅱ protein were 70%. 6% and 76. 5%in PVR C membranes, and 73.9% and 69.6%in PVR D membranes respectively. Relationship between positive expression and membranes＇ grades appeared no statistical correlation （P 〉 0. 05）. Statistical analysis showed that there was a correlation between the expression of CTGF and TGF-βR Ⅱ , FN,and collagen Ⅰ and Ⅲ protein,respectively. Conclusions The expression of CTGF and TGF-βR Ⅱ protein is up-regulated in PRM of PVR,which suggests that the activation of TGF-βR Ⅱ is involved in the production of CTGF,and CTGF is closely related to the production of ECM and play an important role in the pathogenesis of PVR.

关 键 词：玻璃体视网膜病 增生性 结缔组织 生长物质 受体 转化生长因子Β 细胞外基质 免疫组织化学 

分 类 号：R774.1[医药卫生—眼科] R446-39[医药卫生—临床医学]