结核分枝杆菌ESAT-6与CFP-10蛋白间的相互作用  

Protein-protein Interaction Between ESAT-6 and CFP-10 of Mycobacterium Tuberculosis

作　　者：李岩[1] 鲍朗[1] 张会东[1] 王晓樱[1] 朱海龙[1] 李娅莎[1] 

机构地区：[1]四川大学华西基础医学与法医学院感染免疫研究室,成都610041

出　　处：《四川大学学报（医学版）》2006年第3期349-352,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30271172)资助

摘　　要：目的观察结核分枝杆菌ESAT-6与CFP-10蛋白间的相互作用。方法采用PCR技术克隆结核分枝杆菌ESAT-6与CFP-10基因,构建酵母双杂交载体质粒pGBKT7-ESAT-6和pGADT7-CFP-10,经酶切分析和DNA测序证实连接片断的正确性后,采用醋酸锂法顺序转染酵母菌AH109。结果共转染pGBKT7-ESAT-6和pGADT7-CFP-10的酵母菌AH109可以在SD/-Ade/-His/-Leu/-Trp培养基上生长,β-半乳糖苷酶活性实验阳性。结论结核分枝杆菌ESAT-6与CFP-10蛋白可以相互作用。Objective To investigate the protein-protein interaction between ESAT-6 and CFP-10 of mycobacterium tuberculosis. Methods ESAT-6 gene and CFP-10 gene were amplified from mycobacterium tuberculosis genome DNA. The ESAT-6 gene was subcloned into pGBKT7 and the CFP-10 gene was subcloned into pGADT7-Rec. After being verified with restriction endonuclease digestion and DNA sequencing, the recombinant vectors were transformed into yeast cell AH109 by lithium acetate method. Results The yeast cells co-transformed with pGBKT7-ESAT-6 and pGADT7-CFP-10 grew on SD/-Ade/-His/-Leu/-Trp plates, and galactosidase activity assays showed positive results. Conclusion ESAT-6 and CFP-10 protein could interact with each other in yeast cells.

关 键 词：ESA-6 CFP-10 酵母双杂交 蛋白间相互作用 结核分枝杆菌 

分 类 号：R378.911[医药卫生—病原生物学]