CAGE基因在颅脑肿瘤中的表达研究  

Research on the Expression of Cancer Associated Gene in the Intracranial Tumors

作　　者：高飞[1] 由田[2] 吕晶玉[3] 王运杰[1] 

机构地区：[1]中国医科大学第一临床学院神经外科,沈阳110001 [2]中国医科大学第一临床学院急诊科 [3]中国医科大学医学遗传学教研室

出　　处：《四川大学学报（医学版）》2006年第3期424-426,共3页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨肿瘤相关基因(CAGE)在颅脑肿瘤发生发展中的意义及其应用价值。方法采用半定量RT-PCR方法,检测8种共17例正常组织,35例脑膜瘤和63例胶质瘤组织CAGE基因的表达。结果CAGE基因在除睾丸以外的正常组织中不表达;4例脑膜瘤(11.3%)和57例胶质瘤(90.5%)中CAGE基因表达阳性,该基因在两类肿瘤中表达差异有统计学意义(P<0.001)。胶质瘤级CAGE基因表达量为内对照的0.5±0.11倍,级为0.86±0.23倍,级为1.85±0.37倍,级为2.7±0.46倍,基因表达量与病理组织学分级呈正相关(r=0.82)。结论CAGE在脑胶质瘤细胞转化和癌变过程中起着重要作用,它可能成为胶质瘤的肿瘤特异性抗原。Obiective To investigate the function significance and application value of cancer associated gene （CAGE） in the pathogenesis of intracranial tumors. Methods Semi-quantitative RT-PCR was performed to detect the expression of CAGE gene in 17 normal tissues, 35 meningioma cases and 63 glioma cases. Results The CAGE gene was expressing in none of the normal tissues except testis, but in-4 meningioma （11.3%） and 57 glioma cases （90. 50%）. The expression of CAGE gene showed significant difference between the meningioma and glioma （P〈0. 001/. The amount of CAGE mRNA was 0. 5±0. 11, 0. 86±0. 23, 1.85±0. 37 or 2.7±0. 46 times of the internal control one in glioma grade Ⅰ ,Ⅱ , Ⅲ or Ⅳ respectively. The expression of CAGE gene increased with the pathological grade（r=0. 82）. Conclusion The CAGE plays important role in the pathogenesis of glioma, and may act as the tumor specific antigen for glioma.

关 键 词：CAGE基因 RT—PCR 胶质瘤 肿瘤免疫 

分 类 号：R739.4[医药卫生—肿瘤]