内源性光动力疗法对人结肠癌SW480细胞cAMP和cGMP的影响  

Effects of Endogenous Photodynamic Therapy on Intracellular cAMP and cGMP Concentrations of Human Colon Carcinoma Cell Lines SW480

作　　者：郑江华[1] 时德[1] 陈祖林[2] 肖卫东[2] 梁平[2] 

机构地区：[1]重庆医科大学附属第一医院血管外科,重庆市普外科学重点实验室,重庆400016 [2]第三军医大学附属新桥医院普外科,重庆400037

出　　处：《中国普外基础与临床杂志》2006年第3期271-274,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金资助项目(编号:30271481)~~

摘　　要：目的观察内源性光动力疗法(photodynamictherapy,PDT)对人结肠癌SW480细胞环磷酸腺苷(cAMP)和环磷酸鸟苷(cGMP)的影响。方法将SW480细胞分为4组:对照组、光照组、δ氨基酮戊酸(ALA)组、内源性PDT(ALA PDT)组,用放射免疫法检测光照后30、60、90及120min各组细胞的cAMP和cGMP的浓度。结果cAMP浓度在监测时间内,ALA PDT组在30min时最高,明显高于其余各组相应时相及同一组内其余时相(P均<0.001);在60、90及120min时与其它各组相应时相比较,差异均无统计学意义;其余各组在监测时间范围内,组间及组内不同时相间差异均无统计学意义。cGMP浓度在各组细胞的组间及同组内不同时相间差异均无统计学意义。结论内源性PDT在杀伤肿瘤细胞的同时,SW480细胞也能通过细胞内cAMP浓度的瞬时升高产生保护作用,抑制细胞凋亡。Objective To investigate the effects of endogenous photodynamic therapy （PDT） on intracellular cAMP and cGMP concentrations of human colon carcinoma cell lines SW480. Methods SW480 cells were divided into control group, light group, 8-aminolevulinic acid （ALA） group （ALA group） and endogenous PDT group （ALA-PDT group）. Intracellular cAMP and cGMP concentrations of each group were detected by radioimmunoassay at 30, 60, 90 and 120 min after irradiation. Results There was a significant increase in intracellular cAMP concentration of ALA-PDT group at 30 min after irradiation （P〈0. 001） and sequent decrease, but intracellular cAMP concentrations of ALA-PDT group at 60, 90 and 120 min after irradiation had no statistical difference than the other groups （P〉0.05）. Intracellular cGMP concentration of different time point of each group was not significantly different. Conclusion These results indicate that the cytoprotection of SW480 cell are produced by an instantaneous increase in the intracellular cAMP concentration while endogenous PDT is killing SW480 cell.

关 键 词：内源性光动力疗法 SW480细胞 环-磷酸腺苷 环-磷酸鸟苷 

分 类 号：R735.35[医药卫生—肿瘤]