egfr基因两个重要片段和核基质结合  

Two Important egfr Fragments Are Found to Be Attached to Nuclear Matrix

作　　者：李冠武[1] 王云[1] 柏素云[2] 温博贵[1] 

机构地区：[1]汕头大学医学院病理系/肿瘤分子生物学开放实验室/生物化学教研室,广东汕头515041 [2]泰山医学院生化教研室,山东泰安271000

出　　处：《癌变．畸变．突变》2006年第3期198-200,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：广东省卫生厅项目(No:A2001418);李嘉诚汕头大学研究发展基金资助

摘　　要：背景与目的:探讨舌鳞癌细胞株TCA8113中egfr基因两个重要片段和核基质的关系。材料与方法:应用免疫组化和Western印迹对TCA8113中表皮生长因子受体(Epidermalgrowthfactorreceptor,EGFR)的表达进行检测,通过PCR扩增了解egfr基因的两个片段与核基质的结合关系。结果:TCA8113不仅表达EGFR,而且,在该细胞株中EGFR基因的两个片段(一个为由829bp长的编码区片段和110bp长的内含子构成的939bp长的片段;另一个为110bp长的非编码区片段)均与核基质紧密结合。结论:TCA8113细胞中egfr基因通过这两个片段与核基质紧密结合,并可能通过这种相互作用调控EGFR的表达。进一步分离和鉴定与egfr基因片段结合的核基质蛋白,并研究它的功能,对了解EGFR与肿瘤发生发展的关系,以及阻断EGFR治疗肿瘤有一定意义。BACKGROUND ＆ AIM：To explore the relationship between two fragments of egfr with the nuclear matrix （NM） in tongue squamous carcinoma cell line TCA8113.MATERIAL AND METHODS： The expression of EGFR was evaluated in this cell line by immunohistochemistry and Western Blot ,and the relationship between two fragments of egfr with NM was studied by PCR. RESULTS： TCA8113 expressed EGFR. egfr bound to NM tightly by two gene fragments （a 110 bp non-coding fragment, and a 940 bp fragment made up by a 829 bp expression fragment and a 110 bp intron）. CONCLUSION： egfr gene could attach to the nuclear matrix by these two fragments which may be involved in the regulation of egfr gene expression in TAC 8113 cells. The function of these two fragments binding to nuclear matrix protein, the relationship between EGFR and tumor development ＆ using anti-EGFR as a therapy should be explored.

关 键 词：表皮生长因子受体 核基质 舌鳞癌 

分 类 号：R73[医药卫生—肿瘤]