乳腺癌组织中p51基因的表达及其意义  

Expression and Significance of P51 Gene in Breast Cancer

作　　者：杨开颜[1] 俞康[1] 李剑敏[1] 黄卡特[1] 周韧[2] 

机构地区：[1]温州医学院附属第一医院,浙江温州325003 [2]浙江大学医学院,浙江杭州310031

出　　处：《癌变．畸变．突变》2006年第3期249-251,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：温州市科技发展计划项目(No.S2001A29)

摘　　要：背景与目的:研究p51基因与乳腺癌的关系。材料与方法:应用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记(Terminaldeoxynucleotidyltransferasebiotin-dUTPnickendlabeling,TUNEL)、RT-PCR等技术检测乳腺癌组织p51基因的表达及其与细胞凋亡、激素受体的关系。结果:乳腺癌(45例)组织p51基因表达(46.7%)高于良性乳腺病变(35例)组织(11.4%);淋巴结转移阳性者p51基因表达(90.5%)明显高于阴性者(8.3%);p51基因表达与雌激素、孕激素受体无相关性(P>0.05);p51基因表达阳性的肿瘤细胞凋亡率与阴性细胞差异无统计学意义(P>0.05)。结论:P51基因表达增多可能与乳腺癌发病及转移有关。BACKGROUND ＆ AIM： To investigate the changes of p51 gene in breast cancer. MATERIAL AND METHODS： The expression of p51 gene in breast cancer tissues was detected by Terminal Deoxynucleotidyl Transfemse Biotin-dUTP Nick End Labeling（TUNEL） and reverse transcriptase-PCR（RT-PCR）, and the relationship between p51 gene and apoptosis and hormone receptors was analysed. RESULTS： The expression of p51 gene was significantly higher in breast cancer（21/45） than that in benign breast tumors（4/35） （P=0.001 ）.It was also was significantly higher in of lymph nodes positive metastasis（19/21） than in negative ones（2/24） （P=0.006） .The expression of p51 gene is notrelated with ER（＂ER positive＂13/27,＂ER negative＂8/18 P=0.807） ,PR（＂PR positive＂ 11/23,＂PR negative＂ 10/ 22 P = 0.873）;The apoptosis rate of p51 gene positive cells（9.73 ± 1.54） was not significantly different from the negativeones （9.90± 1.36）（P 〉 0. 05）. CONCLUSION： The increasing expression of p51 gene may be related to breast cancer and its metastatic ability.

关 键 词：P51基因 乳腺癌 凋亡 末端脱氧核苷酸转移酶介导的dUTP缺口末端标记 反转录酶-PCR 

分 类 号：R737.9[医药卫生—肿瘤]