银杏叶多糖的分离纯化及鉴定  

The Isolation, Purification and Determination of Polysaccharide from Leave of Ginkgo Biloba L

作　　者：黄桂宽[1] 李毅[1] 谢荣仿 杜所昌[1] 赵农[1] 

机构地区：[1]广西医科大学化学教研室,南宁530021

出　　处：《中国生化药物杂志》1996年第4期157-159,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：银杏叶经水提、醇沉等得到银杏叶多糖（ＬＧＢＰ）。将ＬＧＢＰＨ Ｓｅｐｈａｄｅｘ Ｇ１００层析柱，分离得到ＬＧＢＰ－Ａ和ＬＧＢＰ－Ｂ两种多糖，经电泳与凝胶柱柱层析鉴定均为单一多糖。平均分子量分别为１１×１０＾４和２×１０＾４。经纸层析与高效液相色谱证实其单糖组成分别是：ＬＧＢＰ－Ａ：Ｄ－葡萄糖，Ｌ－鼠李糖、Ｄ－木糖；ＬＧＢＰ－Ｂ：Ｄ－葡萄糖。The Polysaccharide from the leave of Ginkgu bitoba L (LGBP) was obtained by extracting and precipitating. LGBP-A and LGBP-B were isolated by Sephadex G100 gel filtration, which were determined to be chemically homogeneous by means of electrophoresis and gel column chromatogra-phy. The average MW was found separately to be 110 000 and 20 000. The chemical composition was separately LGBP-A; D-glucose, L-rhamnose,D-xylose; LGBP-B: D-glucose by means of high performance liquid chromatogrophy and paper chromatogrophy.

关 键 词：银杏叶 多糖 分离纯化 鉴定 

分 类 号：R914.1[医药卫生—药物化学]