原油降解菌丝状真菌的筛选及降解性能研究  

Screening and Degradability of Crude Oil-degrading Filamentous Fungi

作　　者：沈薇[1] 杨树林[1] 宁长发[1] 

机构地区：[1]南京理工大学生物工程研究所,南京210094

出　　处：《环境科学与技术》2006年第5期11-13,共3页Environmental Science & Technology

基　　金：南京市科学技术局资助项目(200301045)

摘　　要：从南京炼油厂原油污染的土壤中筛选得到2株高效降解原油的菌株,OBD-LM2和OBD-HQM,经初步鉴定菌株OBD-LM2为木霉属,菌株OBD-HQM为曲霉属。原油液体培养基中添加N、P营养盐、温度为25～35℃、盐度<3%时7d菌株OBD-LM2原油降解率为40%～68%,菌株OBD-HQM为35%～55%。采用GC、GC/MS对两株霉菌的原油降解前后组分进行测定,结果表明:菌株OBD-LM2的原油组分降解范围为C11～C28并有许多种类的短链烃(C10)产生,菌株OBD-HQM的原油降解范围仅为C11～C20。Two high efficient oil-degrading strains OBD-LM2 and OBD-HQM was screened from crude oil contaminated soil of a refinery, with OBD-LM2 identified as Trichoderma sp and OBD-HQM as Aspergillus sp. Degradability of OBD-LM2 ranged in 40%~68% and OBD-HQM ranged in 35%~55% at the condition of nutrients addition in the culture base, temperature of 25~35℃, 7d, salinity less than 3%. GC and GC/MS of crude oil before and after biodegradation showed that degradation by OBD-LM2 ranged in C11~C28 and OBD-HQM in C11~C20.

关 键 词：原油污染 生物降解 降解特性 

分 类 号：X17[环境科学与工程—环境科学]