四膜虫彗星实验在环境水质检测中的应用  

Evaluation of Environmental Water Quality by Application of Comet Assay with Unicellular Protozoan Tetrahymena thermophila

作　　者：王丽[1] 鲁志松[1] 丁书茂[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院环境科学实验室,武汉430079

出　　处：《环境科学与技术》2006年第5期31-33,共3页Environmental Science & Technology

基　　金：国家科技部"十五"科技攻关计划基金(2001BA704B01)

摘　　要：介绍四膜虫作为生物材料在彗星实验水质检测中的应用,探讨了将单细胞原生生物应用于彗星实验在环境水质检测、遗传毒性检测等方面的应用优势和发展前景。研究结果表明:过氧化氢浓度与细胞损伤之间呈现良好的剂量-效应关系,东湖水和长江水未造成四膜虫DNA的损伤,印染厂污水对细胞有严重的损伤作用,可能会影响正常的生态环境。Unicellular protozoan Tetrahymena thermophila was used in comet assay to detect DNA damage induced by wastewater so as to evaluate water quality. Results showed that a fine dosages-response relation between H2O2 and DNA damage was presented. Water from East Lake and the Yangtze River did not induce visible DNA damage while effluent from a printing and dyeing plant induced significant DNA damage which could impact on normal ecoenvironment.

关 键 词：分子生物学 彗星实验 原生生物 四膜虫 水质检测 

分 类 号：X830.2[环境科学与工程—环境工程]