发根农杆菌转化茶树的研究  

Study on Agrobacterium-Mediated Transformation of Camellia sinensis L.

作　　者：彭正云[1] 刘德华[1] 肖海军[1] 蒋立文[1] 张丽霞[2] 向勤锃[2] 唐道方[1] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]山东农业大学园艺学院,山东泰安271018

出　　处：《湖南农业大学学报（自然科学版）》2006年第2期190-194,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省教育厅项目(63020152)

摘　　要：以3个品种茶树的不同部位、器官为材料,探讨发根农杆菌转化茶树及毛状根增殖的条件.感染1025菌种的大红品种茎段,在添加IBA 0.4mg/L的1/2MS培养基上诱导出了毛状根.茶树毛状根细如头发,着生稀疏长根毛,具有激素自主性、分枝多、生长快等特点.经正交试验,将1/2MS培养基的KNO3,NH4NO3质量浓度降至MS培养基的1/8时的培养基,是毛状根伸长、分枝较好的基本培养基.添加IBA 0.2mg/L的培养基,毛状根的伸长、分枝优于添加IBA 0.1mg/L的培养基,但在毛状根上出现少量愈伤组织.Different positions and organs of three varieties of tea plant for materials are used to research the condition of Agrobaterium-mediated transformation of Camellia sinensis L. and hairy roots multiplication. The result shows： Hairy roots were obtained after infection of Dahong stems cultured on 1/2MS culture adding IBA 0.4 mg/L with Agrobacterium rhizogenes strains 1025; and the hairy roots with the features of thinner like hair, whit more paucity and longer root hair, having hormone independence, much ramification, fast increment. Meanwhile, through the orthogonal test, finding hairy roots grow well after inducing content of KNO3, NH4NO3 of 1/2MS to 1/8MS, and hairy roots elongate and ramify on the culture adding IBA 0.2 mg/L more than that of adding IBA 0.1 mg/L, but the latter led to more callus.

关 键 词：茶树 发根农杆菌 毛状根 转化 

分 类 号：S571.101[农业科学—茶叶生产加工]