气相色谱-质谱法测定猪肉中的莱克多巴胺  

Determination of Ractopamine Residues in Swine Muscle with GC-MS

作　　者：应永飞[1] 陆春波[1] 任玉琴[1] 林仙军[1] 吴平谷[2] 

机构地区：[1]浙江省畜产品质量安全检测中心,浙江杭州310020 [2]浙江省疾病预防控制中心,浙江杭州310020

出　　处：《中国兽药杂志》2006年第5期23-26,共4页Chinese Journal of Veterinary Drug

摘　　要：建立了气相色谱-质谱法（GC-MS）测定猪肉中莱克多巴胺残留的方法.用乙腈提取试样中的莱克多巴胺,经SLH固相萃取柱净化,氮气吹干,衍生化后进行气相色谱-质谱法测定.猪肉中莱克多巴胺的检出限为0.5μg/kg,定量限为2.0μg/kg.溶液中莱克多巴胺浓度在0.02～1.0μg/mL范围内,峰面积与之呈良好的线性关系.平均回收率在81.4%以上,批内相对标准偏差小于8.4%.In this paper, a new Gas Chromatography-Mass Spectrometry （GC-MS）method for determination of ractopamine residues in swine muscle was reported. The drug was extracted with acetonitrile from muscle. The extraction was evaporated （40℃） and residue was dissolved in ethyl acetate, purified by passing through SLH solid-phase extraction （SPE） cartridges. The elution was evaporated by nitrogen blow and derivatizated with BSTFA, and then assayed by GC-MS. The limit of detection （LOD） was 0.5 μg/kg, and the limit of quantitation （LOQ） was 2.0 μg/kg. The calibration curve was linear between 0.02 μg/mL and 1.0 μg/mL for ractopamine. Recovery of ractopamine was above 81.4%, and intra-relative standard deviation （n = 5） were less than 8.4%.

关 键 词：固相萃取 气相色谱-质谱法 莱克多巴胺 残留量 猪肉 

分 类 号：S859.84[农业科学—临床兽医学]