用PCR-SSCP方法检测TLR9基因多态性  

Detecting TLR9 gene polymorphism with polymerase chain reaction-single strand conformation polymorphism

作　　者：张合喜[1] 邹立君[2] 王友洁[2] 吕斌[2] 周宜开[2] 

机构地区：[1]新乡医学院公共卫生学系,河南新乡453003 [2]华中科技大学同济医学院公共卫生学院,湖北武汉430030

出　　处：《新乡医学院学报》2006年第3期217-219,共3页Journal of Xinxiang Medical University

基　　金：国家自然科学基金重大项目(NO.39990570)

摘　　要：目的 探讨用多聚酶链反应-基因单链构象（PCR-SSCP）方法研究Toll样受体基因多态性。方法 用PCR-SSCP方法检测82例肿瘤患者和82例健康对照人群的TLR9基因-1486、-1237、1174、2848位点的单核苷酸多态性。结果 164例人群样本中，TLR9基因多态性频率分布分别是：1486、1174、2848位点在对照组和病例组的比例为0％和1．2％，12％和7．3％，0％和37％；-1237在对照组和病例组中均未发现多态性。在病例组中出现4种多态性的比例为12．2％，在对照组中为12％，P=0．0092，OR=11．25，95％CI为1.41～90．05。结论 TLR9多态性在人群中与癌肿有一定的关系，基因突变可能导致癌肿危险度增加。Objective To explore TLR9 gene polymorphism with polymerase chain reaction - single strand conformation polymorphism（PCR SSCP）. Methods single nucleic- acid polymorphism （SNPs, -1468, -1237, 1174 and 2848 ） of TLR9 gene were screened by PCR- SSCP in 82 tumour cases and 82 matched controls. Results The TLR9 gene was measured in 164 blood samples from Hubei （including 82 tumour cases and 82 health controls）. The frequencies of TLR9 gene SNPs： the proportions at - 1486, 1174 and 2848 sites between controls and cases were 0% vs 1.2%, 1.2% vs 7. 3%, 0% vs 3.7% ,respectively. At - 1237 site of TLR9 gene,, we did not screen polymorphism in controls and eascs. The occurred possibility of one of 4 SNPs in cases was 12.2 % but 1.2 % in controls （P = 0. 0092, OR = 11.25, 95 % CI 1.41-90.05）. The incidences of four SNPs in 164 samples were0.6%, 0%, 4.3% andl.8% ,respectively. Conclusion In Hubei, China population, there may be some relations between TLR9 gene polymorphism and tumor. The mutations of TLR9 gene can increase the risk of cancer.

关 键 词：TOLL样受体9 基因多态性 易感性 

分 类 号：R73-31[医药卫生—肿瘤]