实验性大鼠重症急性胰腺炎早期肺组织中MDA、MPO的变化  

The change of pulmonary MDA and MPO induced by early-stage severe acute pancreatitis in rats

作　　者：王明海[1] 张义胜[1] 赵国海[1] 史海生[1] 蒋勇[1] 陶坤[1] 韩光峰[1] 吴敬医[1] 

机构地区：[1]皖南医学院附属弋矶山医院普外四科,安徽芜湖241001

出　　处：《皖南医学院学报》2006年第2期81-83,共3页Journal of Wannan Medical College

基　　金：安徽省教育厅自然科学研究基金项目(2004kj346)

摘　　要：目的:探讨重症急性胰腺炎在早期时肺组织中MDA、MPO的变化与意义。方法:健康成年雄性SD大鼠40只,按随机化原则分成5组,每组8只大鼠。即假手术组、重症急性胰腺炎30、60、120和360min组(SAP组:胰胆管逆行注入5%牛磺胆酸钠溶液),剖杀各组大鼠,分别检测肺组织中丙二醛含量(MDA)和髓过氧化物酶(MPO)活性。结果:假手术组动物肺组织MDA为(1.61±0.62)nmol/mg,MPO为(2.63±0.74)U/g。重症急性胰腺炎模型制成后30、60、120和360min大鼠肺组织MDA和MPO均升高,分别为(1.91±0.94)nmol/mg、(2.17±0.64)nmol/mg、(2.54±1.14)nmol/mg、(4.99±1.68)nmol/mg和(3.03±0.83)U/g、(3.88±1.08)U/g、(4.57±1.16)U/g、(6.43±1.15)U/g。在60min时MPO明显升高(P<0.05);在360min时MDA显著升高(P<0.01)。结论:试验性大鼠重症急性胰腺炎早期(360min)时,肺组织中出现脂质过氧化反应和大量中性粒细胞浸润。Objective ：To investigate the changes and signifieanee of pulmonary MDA and MPO induced by severe acute pancreatitis during its early stage. Methods ： Forty adult male Sprague-Dawley rats weighting 300-400g were randomly divided into five groups with 8 ones in each sham-operation group, SAP 30,60,120 and 360-minute group. The models of SAP were induced by retrograde injection of 5 % sodium taurocholate into the biliopancreatic duct of rats. MDA levels and MPO activity in pulmonary tissue were measured by dissecting each rat at a different time interval. Results ： Pulmonary MDA and MPO levels in the sham group were （1.61± 0.62）nmol/mg and （2.63 ± 0.74） U/g, respectively. After the model of SAP were induced, pulmonary MDA and MPO levels increased at the phase of 30,60,120 and 360 minute. They were（1.91 ± 0.94） ritual/rag, （2.17 ± 0.64） nmol/mg, （2.54 ± 1.14） nmol/mg, （4.99 ± 1.68） nmol/mg and （ 3.03 ± 0.83 ） U/g, （ 3.88 ± 1.08）U/g,（4.57± 1.16）U/g,（6.43 ± 1.15）U/g, respectively. MPO levels were increased significantly at 60 minute （P 〈 0.05） while MDA levels were seen significant increase at 360 minute（ P 〈 0.01 ）. Conclusion ：Lipid peroxidation and much PMN infiltration exist in pal- monary tissue during SAP early stage in rats.

关 键 词：重症急性胰腺炎 急性肺损伤 丙二醛 髓过氧化物酶 

分 类 号：R576[医药卫生—消化系统]