不结球白菜叶片壳聚糖酶的理化特性研究  

Studies on chemical and physical characters of chitosanase from non-heading Chinese cabbage leaves

作　　者：陈惠萍[1] 徐朗莱[1] 

机构地区：[1]南京农业大学生命科学学院

出　　处：《海洋科学》2006年第5期36-39,共4页Marine Sciences

摘　　要：通过不结球白菜叶片壳聚糖酶对壳聚糖的降解,研究了温度、pH、时间及金属离子等对此壳聚糖酶的影响。结果表明,壳聚糖酶的最适温度为45℃,最适pH为5.5,酶促反应速度在6 h内达到最大值;Mn2+和Cu2+对壳聚糖酶促反应分别起到促进和抑制的作用。经薄板层析分析证实,壳聚糖酶降解壳聚糖为低聚物。The effects of temperature, pH, time and metal ion on chitosanase activity from non heading Chinese cabbage （Brassica campestris L. ssp. chinensis（L. ） Makino） leaves were investigated. The results show that the optimal temperature and pH are 45~C and 5, 5 in degrading chitosan respectively. Otherwise, analysis of thin board chromatograph revealed that chtiosan was decomposed into ehitooligosaccharedes by chitosanase.

关 键 词：不结球白菜 壳聚糖酶 最适温度 最适PH 低壳聚糖 

分 类 号：Q945[生物学—植物学]