甲基丙烯酸环氧丙酯致人胚肺成纤维细胞恶性转化的研究  

Studies on Malignant Transformation of Human Embryonic Lung Fibboblasts Induced by Glycisyl Methacrylate

作　　者：王媛[1] 谢大英[1] 许建宁[1] 李忠生[1] 贺锡雯[1] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所

出　　处：《基础医学与临床》1996年第3期207-210,共4页Basic and Clinical Medicine

基　　金：自然科学基金

摘　　要：本实验对经典的细胞转化实验进行了改进，应用甲基丙烯酸环氧丙酯（简称ＧＭＡ）多次作用，建立了人胚肺成纤维细胞转化系统。观察了人胚肺成纤维细胞恶性转化过程中细胞形态和原癌基因ｃ－ｍｙｃ的改变。结果表明：在ＧＭＡ的多次作用下，细胞的恶性程度逐渐增高，形态上由正常到开始排列紊乱，最终形成大量细胞致密堆集的转化灶。经多次染毒后形成的转化细胞在含３％小牛血清的培养基中生长旺盛，其原癌基因ｃ－ｍｙｃ的ｍＲＮＡ表达水平显著增高。本文探讨了人细胞形态及基因的改变与细胞转化乃至肿瘤发生的关系。e have observed the changes of the morphology and proto-oncogene,c-myc,during themalignant transformation of human embryonic lung fibroblasts induced by glycidyl methacrylate(GMA).The results showed that with the continuous effects of GMA the malignant degree ofcells enchanced,their arrangernent become chaotic,and finally they formed lots of transformedclonies.The transformed cells could grow very well in the culture medium with 3% FBS,mean-while,the expression of c-myc increased significantly.The purpose of this study is to discuss therelationships between the changes of morphology/proto-oncogene and the transformation of cells,dnd then the oncology of cancer.

关 键 词：人 肺成纤维细胞 细胞转化 甲基丙烯酸 环氧丙酯 

分 类 号：R730.231.1[医药卫生—肿瘤]