抗坏血酸提高月季切花失水胁迫耐性与增加APX活性的关系  

Ascorbate Acid Increases Tolerance to Water Deficit Stress in Flowers of Cut Roses(Rosa hybrida L.) Caused by Enhanced Ascorbate Peroxidase Activity

作　　者：金基石[1] 李永红[2] 单宁伟[1] 高俊平[1] 

机构地区：[1]中国农业大学观赏园艺与园林系,北京100094 [2]深圳职业技术学院应用化学与生物技术学院,深圳518055

出　　处：《园艺学报》2006年第2期333-337,共5页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(39870490)

摘　　要：以‘Samantha’品种为试材,确定了适宜的抗坏血酸(AsA)预处理浓度,探讨了AsA和抗坏血酸过氧化物酶(APX)抑制剂(对氨基酚,βaminophenol)预处理对花瓣中相对电导率、AsA含量以及APX活性的影响。与未经失水胁迫的切花月季相比较,失水胁迫处理抑制切花的开放进程,缩短了瓶插寿命。AsA1000mg·L-1预处理可有效地改善花朵的开放状况,显著降低花瓣中相对电导率的增加,明显提高花瓣中AsA的含量和APX活性。500mg·L-1βaminophenol预处理则起到相反的效应。结果说明,AsA对‘Samantha’月季切花失水胁迫耐性的改善与花瓣中APX活性的提高相联系。Pretreatment with ascorbic acid （AsA） increased tolerance of the flowers of cut roses to water deficit stress. The optimum pretreatment concentrations of AsA and β-aminophenol （ an inhibitor of ascorbate peroxidase, APX） were determined in cut rose ‘Samantha＇ flowers. Water deficit stress inhibited flower opening and reduced vase life. Pretreatment of 1 000 mg·L^-1 AsA improved markedly the flower opening, delayed significantly the increase in relative electric conductivity, and increased obviously AsA content and APX activity. The negative results were obtained when 500mg·L^-1 β-aminophenol pretreatment was used. These results indicate that the improvement of AsA on tolerance to water deficit stress may be related to enhanced APX activity in petals of cut roses.

关 键 词：月季 切花 失水胁迫耐性 抗坏血酸 抗坏血酸过氧化物酶 

分 类 号：S685.12[农业科学—观赏园艺]