莴苣高效瞬时表达体系的建立  

A Highly Efficient System Establishment of Transient Expression in Lettuce

作　　者：李静[1] 陈敏[2] 刘现伟[2] 沈法富[1] 王鹏[2] 

机构地区：[1]山东农业大学农学院 [2]山东大学生命科学学院

出　　处：《园艺学报》2006年第2期405-407,共3页Acta Horticulturae Sinica

基　　金：山东省优秀中青年奖励基金资助项目(2005BS02015)

摘　　要：以GUS基因作为报告基因,利用农杆菌真空渗透法,采取正交试验设计对莴苣(LactucasativaL.sp)的瞬时表达条件进行研究,建立了高表达水平的瞬时转化体系(乙酰丁香酮200μmol/L、菌液密度OD600值0.8、真空处理30min、共培养6d)。应用结果表明:优势组合的表达水平比对照的表达水平提高16.3倍。Using Agrobacterium vacuum infiltration method the plant bivalent expression vector pBI121 containing GUS as a report gene was transformed into lettuce （Lactuca sativa L. sp）. Through orthogonal experiment, a highly efficient system of transient expression was observed in plant intact leaves when they were treated with 200 μmol/L acetosyringone, 0. 80D600 bacterium density, vacuum 30 min and co-culture 6 days. Transgenic expression level of the optimal condition is improved 16. 3 times than that of the control.

关 键 词：莴苣 农杆菌 真空渗透 GUS 瞬时表达 

分 类 号：S636.2[农业科学—蔬菜学]