1800MHz射频电磁场对人乳腺癌细胞蛋白质表达的影响  

Effects of GSM 1800 MHz radiofrequency electromagnetic fields on protein expression profile of human breast cancer cell MCF-7

作　　者：曾群力[1] 翁瑜[1] 陈光弟[1] 鲁德强[1] 姜槐[1] 许正平[1] 

机构地区：[1]浙江大学医学院浙江省生物电磁学重点研究实验室,杭州310031

出　　处：《中华预防医学杂志》2006年第3期153-158,共6页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金资助项目(50137030;30170792);教育部科学技术研究重点资助项目(104092);浙江省自然科学基金资助项目(301524);浙江省卫生厅重点资助项目(2004ZD006)

摘　　要：目的采用高通量的蛋白质组学技术研究人乳腺癌细胞株MCF7细胞受GSM1800MHz射频电磁场辐照后,其蛋白质表达谱的变化,以探索移动电话信号对细胞正常生理功能的可能影响。方法比吸收率为3.5W/kg时,采用不同时间(1、3、6、12和24h)和辐照模式(间断辐射和连续辐射)的GSM1800MHz射频电磁场处理MCF7细胞后,直接抽提蛋白质,然后进行双向凝胶电泳。凝胶经银染后,使用PDQuest软件分析假辐照组与电磁场辐照组间的差异表达蛋白质斑点。每个实验重复3次。结果凝胶上平均可检测到1100个蛋白斑点。3.5W/kg连续辐射6h未发现差异点,其他暴露情况下均可检测到不同数量的差异蛋白,以3.5W/kg间断辐射3h和连续辐照12h检测的差异蛋白点较多,分别为18个和7个。通过搜索SWISSPROT蛋白数据库,对差异蛋白的类别和功能进行了初步推测,结果表明差异蛋白主要与生物分子的合成和调控、信号转导、DNA损伤修复等功能相关。结论GSM1800MHz射频电磁场对MCF7细胞蛋白质表达谱具有一定程度的影响,且依赖于暴露的强度、时间和模式,影响环节可能涉及多个生物学过程。Objective To study the effects of GSM 1800 MHz radiofrequency electromagnetic fields （RF EMF） exposure on protein expression profile of human breast cancer cell line （ MCF-7 ）, as to exploring the possible effects on normal cell physiological function. Methods MCF-7 ceils were continuously or intermittently （5 minutes field on followed by 10 minutes off） exposed to RF EMF for different duration （1 hour, 3 hours, 6 hours, 12 hours, or 24 hours） at an average specific absorption rate （SAR） of 3.5 W/kg~ The extracted proteins were separated by 2-dimensional electrophoresis and the protein-spot distribution of the sliver-stained gels was analyzed by using PDQuest software 7. 1. Each experiment was repeated three times. Results On the average, around 1100 proteins were detected using pH 4 -7 IPG strip. There were no differential proteins found under continuous exposure at SAR of 3.5 W/kg for 6 hours. Under other exposure conditions, we found various differentially expressed proteins in exposure groups as compared with the sham-exposed controls. Especially in 3 hours intermittent exposure and 12 hours continuous exposure, eighteen and seven differential proteins were detected, respectively. The categories and functions of these differentially expressed proteins were analyzed by searching of SWISS-PROT protein database, which suggested that these proteins should be related to the functions of biosynthesization, signal transduction, and DNA damage and repair. Conclusions Data indicated that the protein expression changes induced by RF radiation might depend on exposure duration and mode. Many biological processes might be affected by RF exposure.

关 键 词：电磁场 蛋白质组 肿瘤细胞培养 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤] R392.3[医药卫生—临床医学]