0.4mT工频磁场对骨骼肌肌质网囊泡游离钙离子转运的影响  

Effects of power frequency magnetic field on Ca 2+ transport of skeletal muscle sarcoplasmic reticulum vesicles

作　　者：刘仁臣[1] 周贞洁[1] 褚克平[1] 刘秀丽[2] 陈树德[1] 夏若虹[1] 

机构地区：[1]上海华东师范大学光谱学与波谱学教育部重点实验室,200062 [2]华东师范大学化学系

出　　处：《中华预防医学杂志》2006年第3期168-172,共5页Chinese Journal of Preventive Medicine

摘　　要：目的探讨工频磁场对骨骼肌肌质网钙转运动力学的影响。方法利用动态光谱法检测0.4mT、50Hz正弦磁场辐照过的骨骼肌肌质网Ca2+转运和钙泵活性,用3H兰尼碱结合实验检测Ca2+释放通道的活性,用荧光偏振法检测肌质网膜的流动性等。结果0.4mT、50Hz正弦磁场辐照导致提取的肌质网囊泡Ca2+摄取初速率由每秒(29.18±3.90)pmol/mg下降到(24.60±3.81)pmol/mg,钙泵的活性由每分钟(0.93±0.05)μmol/mg下降到(0.69±0.07)μmol/mg;导致Ca2+释放初速率由每秒(4.83±0.82)pmol/mg上升到(5.65±0.43)pmol/mg,3H兰尼碱结合度由(1.10±0.12)pmol/mg上升到(1.16±0.13)pmol/mg,分别上升了15%和5%。结论0.4mT工频磁场辐照引起的肌质网Ca2+摄取下调和Ca2+释放上调是由于肌质网钙泵活性降低和Ca2+释放通道活性升高所致。Objective To investigate the effects of power frequency magnetic field on the Ca^2＋. transport dynamics of isolated sarcoplasmic reticulum vesicles. Methods The assays of Ca^2＋ uptake time course and the Ca^2＋ -ATPase activity of sarcoplasmic reticulum vesicles were investigated by using dynamic mode of spectrometry with a Ca^2＋ dye; Ca^2＋ release channel activation was examined by 3H-ryanodine binding and Ca^2＋ release assays; membrane fluidity of sarcoplasmic reticulum vesicles was examined by fluorescence polarization, without or with exposure to the vesicles at a 0. 4 mT, 50 Hz sinusoidal magnetic field. Results 0. 4 mT, 50 Hz sinusoidal magnetic field exposure caused about a 16% decline of the initial Ca^2＋. uptake rate from a （29. 18 ±3.90） pmol · mg^-1 · s^-1 to a （24. 60 ±3.81） pmol · mg^-1 · s^-1 and a 26% decline of the Ca^2＋-ATPase activity from （0.93 ±0.05） μmol ·mg^-1· min^-1 to （0.69 ±0.07） μmol ·mg^-1· min^-1 of sarcoplasmic reticulum vesicles,whereas caused a 15% increase of the initial Ca^2＋ release rate from （4.83 ±0.82） pmol · mg^-1 · s^-1 to （5.65 ±0.43） pmol · mg^-1 · s^-1 and a 5% increase in 3 H-ryanodine binding to the receptor from （ 1.10 ± 0. 12） pmol/mg to （ 1.16 ± 0. 13 ） pmol/mg, respectively. Conclusion The decline of Ca^2＋ -ATPase activity and the increase of Ca^2＋ release channel activity should result in a down-regulation of Ca^2＋ dynamic uptake and an up-regulation of Ca^2＋ release induced by exposing the sarcoplasmic reticulum to a 0. 4 mT, 50 Hz power frequency magnetic field.

关 键 词：电磁场 肌浆网 Ca(2+)转运ATP酶 兰尼碱受体钙释放通道 肌质网膜 

分 类 号：R363[医药卫生—病理学]