人乳腺癌裸鼠移植瘤内淋巴管的形态学特点  

Morphological characteristics of lymphatics in transplantation tumor model of human breast infiltrating duct carcinoma in nude mice

作　　者：杜果城[1] 吴诚义[1] 

机构地区：[1]重庆医科大学附属第一医院普外科,重庆400016

出　　处：《重庆医科大学学报》2006年第3期318-321,共4页Journal of Chongqing Medical University

基　　金：重庆市卫生局科研项目(03-02-074)

摘　　要：目的:观察人乳腺癌裸鼠移植瘤内淋巴管的形态及特点,进而探讨移植瘤内是否存在淋巴管新生。方法:将人乳腺浸润性导管癌组织小块移植于5只BALB/c裸鼠胸部乳垫皮下,建立移植瘤模型,52天时摘取肿瘤。采用HE染色观察移植瘤组织学特征,用VEGFR-3标记淋巴管内皮法光镜下观察毛细淋巴管;应用透射电镜观察移植瘤内毛细淋巴管及内皮细胞超微结构。结果:裸鼠接种部位可见直径约0.6～0.8cm大小的肿块。淋巴管内皮标记法可见肿瘤内有较多染成棕黄色的毛细淋巴管,壁厚、腔细、呈条索样的管状结构,肿瘤周边区似多于中心区。透射电镜下,肿瘤内可见较多形态不规则、壁厚腔细、基底膜常不连续、腔面较多微绒毛、管壁外锚丝甚少的毛细淋巴管,其内皮细胞高尔基复合体及粗面内质网丰富、质膜小泡含量甚少。结论:人乳腺癌裸鼠移植瘤内显示有淋巴管新生。Objective： To observe the morphological characteristics of lymphatics and to investigate the intratumoral lymphangiogenesisin transplantation tumor model of human breast infiltrating duct carcinoma in nude mice. Methods： Small slices of human breast carcinoma tissue were transplanted into 5 nude mice to establish implanted tumor model. HE staining was used to observe the characteristics of thetransplantation tumors. Vascular endothelial growth factor-3 （VEGFR-3） label staining was performed to observe lymphatics,and ultrastructure of lymphatics was observed with transmission electron microscope （TEM）. Results： Tumors were viable in al 1 the mice, and grewup to about 0.6-0.8 centimeters in diameter after 52 days when specimen was collected. Under light microscope, lymphatics were found inall the tumors, and were located seemingly much more in periphery than in the center of the tumors. Under TEM some characteristics ofneogentic lymphatics could be seen, such as thick wall, narrow lumen, changes of organell, etc. Conclusion： Neogentic lymphatics can beseen in transplantation tumor modal of human breast infiltrating duct carcinoma in nude mice.

关 键 词：乳腺癌 移植瘤 淋巴管新生 电镜 裸鼠 

分 类 号：R737.9[医药卫生—肿瘤]