抗肿瘤药物与维拉帕米联合对KBV200细胞P-糖蛋白表达的影响研究  

Investigation of the effect of verapamil and chemotherapeutic agents on the P-glucoprotein of the KBV200 cell line

作　　者：陈鸿雁[1] 王驰[2] 舒艳[3] 陈燕[1] 吕彩凤[1] 余晓燕[1] 沈娜[1] 张潜英[1] 叶玲[1] 蒋纪恺[4] 

机构地区：[1]重庆医科大学附属第一医院耳鼻咽喉科,重庆400016 [2]重庆医科大学基础医学院,重庆400016 [3]重庆医科大学附属儿童医院耳鼻喉科,重庆400016 [4]汕头大学医学院

出　　处：《重庆医科大学学报》2006年第3期369-372,共4页Journal of Chongqing Medical University

摘　　要：目的:探讨6种常用抗肿瘤药物长春新碱、阿霉素、平阳霉素、顺铂、5-Fu、环磷酰胺与维拉帕米联合对KBV200细胞P-糖蛋白表达的影响。方法:以喉癌KBV200细胞株为研究对象,通过流式细胞术检测6种抗肿瘤药物作用KBV200细胞的P-糖蛋白表达以及联合维拉帕米对P-糖蛋白表达的影响。结果:维拉帕米分别与长春新碱、阿霉素、平阳霉素、环磷酰胺联合作用,使KBV200细胞的P-糖蛋白表达下降80.17%、89.69%、19.19%及53.48%。其中维拉帕米与长春新碱、阿霉素作用较强。结论:维拉帕米分别与长春新碱、阿霉素、平阳霉素、环磷酰胺联合作用后,P-糖蛋白的表达水平明显下降,对KBV200细胞耐药性的逆转有一定作用。Objective： To observe the effect of verapamil and 6 chemotherapeutic agents vincristine,adriamycin,bleomycin,cyclophosphamide,5-Fu,cisplatin on the P-glucoprotein （P-gp） of the KBV200 cell line. Methods： Flow cytometer（FCM） assay was used to investigate the expression of P-gp on the KBV200 cell line in the different conditions. Results： Verapamil act with vincristine, adriamycin,pingyangmycin and cyclophosphamide separately decreased expression of P-gp on KBV200 cell line by 80.17%,89.69%,19.19% and 53.48% Among all the combinations, the synergistic effects of varapamil act with vincristine and adriamycine show a better effect. Conclu-sion： Verapamil act with vincristine, adriamycin, pingyangrnycin and cyclophosphamide separately can decrease expression of P-gp on KBV200 cell line significantly, and can reverse the drug resistance of KBV200 cell line.

关 键 词：KBV200细胞株 多药耐药 耐药逆转 P糖蛋白 维拉帕米 

分 类 号：R73[医药卫生—肿瘤]