2-甲氧雌二醇对人子宫内膜癌细胞凋亡作用的实验研究  

Study on the Effect of 2-Methoxyestradiol on Apoptosis in the Endometrial Carcinoma Cell

作　　者：方凤奇[1] 王梅[2] 于佩瑶[1] 李颖[1] 刘基巍[1] 

机构地区：[1]大连医科大学附属第一医院肿瘤科,辽宁大连116011 [2]哈尔滨市第一医院妇产科,黑龙江哈尔滨150001

出　　处：《四川肿瘤防治》2006年第2期86-88,共3页Sichuan Journal of Cancer Control

摘　　要：目的:探讨2-甲氧雌二醇(2-ME)对人子宫内膜癌细胞株KLE细胞凋亡作用的影响。方法:将体外培养的人子宫内膜癌细胞株KLE细胞分为实验组和对照组,其中实验组培养液中加入不同浓度的2-ME,对照组不含2-ME。通过流式细胞仪(FCM)观察KLE细胞的凋亡率及凋亡基因p53和Bcl-2在诱导细胞凋亡中的作用。结果:实验组2-ME浓度为5．0μM-20．0μM时,出现细胞凋亡率升高,与对照组相比有显著性差异(P<0．05),实验组2-ME浓度达2．0μM以上时p53和Bcl-2阳性表达率与对照组相比明显下降(P<0．05)。结论:2-ME对体外培养的人子宫内膜癌KLE细胞株有促进凋亡作用。Objective： To investigate the effect of 2-methoxyestradiol（2-ME） on apoptosis in human endometrial carcinoma cell line（ KLE cells） in vitro. Methods： Endometrial carcinoma cell line（ KLE cells） were divided into study group（ coltured with 2- ME in different concentrations） and control group （c oltured without 2-ME）. Apoptotic rate and expression of tumor suppressor gene p53 and Bcl-2 were detected by flow cytometry（FCM）. Results： The apoptotic rates in study group with 2-ME concentrations between 5 ～20μ M were significantly higher than that in control group（P 〈0. 05 ）. The positive expression rate of p53 and Bcl-2 in study group with the 2-ME beyond 2μM were significantly lower than those in control group （P 〈 0.05 ）. Conclusion： The study suggests that 2-ME can induce the apoptosis of KLE ceils in vitro.

关 键 词：2-甲氧雌二醇 子宫内膜癌 KLE细胞 细胞培养 凋亡 

分 类 号：R737.33[医药卫生—肿瘤] R392.6[医药卫生—临床医学]