大剂量吡哆醇对大鼠睾丸一氧化氮合酶和细胞凋亡的影响(英文)  

Effect of high-dose pyridoxine on nitric oxide synthase and apoptosis of rat testis

作　　者：闫蓓[1] 李积胜[1] 刘亚华[1] 杨烽[1] 

机构地区：[1]武警医学院军事预防医学研究所,天津300162

出　　处：《武警医学院学报》2006年第1期6-9,共4页Acta Academiae Medicinae CPAPF

基　　金：国家自然科学基金资助项目(39870689)

摘　　要：[目的]为探讨大剂量吡哆醇(PN)对睾丸结构和功能损害的分子机制,研究其对睾丸一氧化氮合酶 (NOS)和细胞凋亡的影响。[方法]实验采用吖啶橙荧光染色法显示睾丸细胞DNA和RNA,黄递酶(NADPH- d)组织化学法显示睾丸细胞NOS,采用原位缺口平移技术检测睾丸生精细胞凋亡的数目。[结果]与对照组相比,注射PN 3 d,大鼠睾丸间质细胞NOS反应活性明显增强,睾丸生精细胞凋亡数目(38．41±8．20)较相应对照组(11．39±4．86)明显增多,差异有显著性(P<0．01)。[结论]提示大剂量PN腹腔注射后,大鼠睾丸细胞NOS活性和凋亡细胞数目明显增加,这些变化可能是PN损害睾丸结构和功能的分子机制之一。[Objective] To study the changes of testis nitric oxide synthase （NOS） and apoptosis in high dose pyridoxine （PN） for the understanding of the molecular mechanism of high dose PN injurious action on the development and function oftestis. [Methods] DNA and RNA of testicular cells were stained in situ with nick translation （ISNT） technique.[Results] Comparing with the control guoup, the testes demonstrated only very slight histopathological changes in NOS positive leydig cells after a 3 days PN treatment. The 7 days PN treatment caused obvious decrease in the staining of the testis cellular DNA and RNA, distinctive increase of NOS reactive density under the fluorescent light microscope, and significantly higher number of apoptotic testis spermatogenic cells （38.41 ± 8.20） in comparison with the control group （ 11.39 ± 4.86）, （ P ＜ 0.01 ）. [ Conclusion] It is concluded that high dose of PN treatment could accelerate the apoptosis of testicular cells and the activity of NOS positive cells, which might be one of the molecular mechanisms of the injurious action of high dose PN on the development and function of the testis.

关 键 词：吡哆醇 睾丸 一氧化氮合酶 细胞凋亡 

分 类 号：R917[医药卫生—药物分析学]