实时荧光定量RT-PCR检测食管癌Survivin mRNA的表达  

Detection of Surviving mRNA in esophageal cancer by real-time fluorescence quantitative RT-PCR

作　　者：何晓松[1] 钱志英[1] 何流[2] 周晓明[1] 戴美红[1] 张成阳 

机构地区：[1]江苏省肿瘤医院,江苏省肿瘤防治研究所南京210009 [2]南京市江宁医院,江苏南京211100 [3]盐城市肿瘤医院,江苏盐城224003

出　　处：《临床和实验医学杂志》2006年第1期10-11,共2页Journal of Clinical and Experimental Medicine

基　　金：江苏省卫生厅2003年重点课题(H200332)

摘　　要：目的建立实时荧光定量RT-PCR方法检测SurvivinmRNA的表达。方法用Trizol方法提取食管癌组织总RNA后,将其逆转录为cDNA,建立实时荧光定量RT-PCR方法,检测人食管癌SurvivinmRNA的表达。结果SurvivinmR-NA在癌组织中高度表达,与正常食管粘膜有显著性差异。结论所建立的实时荧光定量RT-PCR方法用于检测检测人食管癌SurvivinmRNA的表达,是一种快速有效、灵敏度高、特异性好的定量检测方法。Objective To establish a real - time fluorescence quantitaltive RT - PCR. Methods Total RNA was extracted with Trizol from the esophageal carcinoma tissues and mRNA was transcribed reversely into eDNA. The real - time quantitative RT - PCR was used to detect the expression of Survivin mR NA, Results The expression of Survivin mRNA in the esophageal carcinoma tissues was significantly higher than that in the notrnal esophageal, tissue, Conclusion Real - time fluorescence quantitative RT - PCR used to detect the expression of Survivin mRNA may be. a reliable means for early diagnosis of esophageal carcinoma.

关 键 词：食管癌 实时荧光定量RT-PCR SURVIVIN MRNA 

分 类 号：R735.1[医药卫生—肿瘤]