Profrin II纳米微粒光动力疗法抑制人结肠癌裸鼠种植瘤的实验研究  

ProfrinII Nanopaticles photodynamic therapy on LoVo human colon cancer xenografts in athymic mice.

作　　者：李镠洋[1] 卿三华[1] 盛新华[1] 巴明臣[1] 

机构地区：[1]南方医科大学附属南方医院普通外科,广东广州510515

出　　处：《临床和实验医学杂志》2006年第5期514-516,共3页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨profrin II纳米微粒的光动力疗法(PDT)对结肠癌的生长抑制作用。方法建立人结肠癌LoVo细胞裸鼠种植瘤模型,经尾静脉注入profrin II纳米微粒(30mg/kg体重),光敏化3 h后半导体激光仪垂直照射肿瘤30 m in(能量密度9 J/cm2),照射后连续观察肿瘤体积,瘤体HE染色病理分析。结果profrin II纳米微粒PDT在治疗后早期产生明显的抑制肿瘤增殖作用,瘤体组织坏死,腺腔样结构解离破坏,微血管坏死,血栓形成,延命率65.2%,体积抑瘤率达72.8%,治疗后期,肿瘤体积仍会缓慢增长,但增长速度明显小于对照组。结论profrin II纳米微粒在PDT治疗中抑制裸鼠结肠种植瘤生长,延长荷瘤裸鼠生存期,但仍不能完全抑制肿瘤生长。Objective To evaluated the inhibitory of proffin Ⅱ nanopaticles photodynamic therapy on LoVo human colon cancer xenografts in athymic mice. Methods LoVo human colon cancer xenograft were established in athymic mic. The animals bearing xenografts were treated 30mg/ kg body weight profrin Ⅱ nanopaticles and 3 h later were irradiated with 9J/cm^2 light from a diode laser. After Profrin I1 nanopaticles PDT, the anti - tumor effect was assessed by messuring tumor valume over a 3 -4weeks period, the morphologic changers were observed by macroscopy and microscopy via the histological examination. Results Compared with the control groups, profrinⅡ nanopaticles - PDT - treated tumor had regressed significantly in earlier period with the inhibititing rate being 72.8% （ P 〈0.05）. In the later period post - PDT, tumors growth resumed with a slower rate. ProfrinⅡ nanopaticles - PDT polonged the survival time in the treated group with（ 38.0 ± 6.0 ） days （ P 〈 0.01 ） ; Extensive damage to tumor tissue was found in the earlier period （7 d） post - PDT, whereas in the later period （21 d） post - PDT, islands of vital - looking tumor cells were observed around the damage tissue. Conclusion ProfrinlI nanopaticles - PDT results in inhibition LoVo colon carcinoma growth in post - PDT earlier period in vivo, and can prolong the survival time of nude mice bearing xenografts significantiy, whereas it seems that profrinlI - PDT could not inhibite the growth of colon tumor completely.

关 键 词：光动力疗法 纳米微粒 结肠 肿瘤 

分 类 号：R735.35[医药卫生—肿瘤] R944.9[医药卫生—临床医学]