中国东北汉族人群WNK4基因多态性与原发性高血压的关联性  

The Relationships between the Polymorphism of WNK4 Gene and Essential Hypertension in North-Eastern Han Population

作　　者：孙志军[1] 杨志勇[1] 李妍[2] 吕晶玉[2] 石景璞[3] 李岭[2] 赵彦艳[2] 

机构地区：[1]中国医科大学附属第二医院心内科,辽宁省沈阳市110004 [2]中国医科大学医学遗传学教研室 [3]中国医科大学流行病学教研室

出　　处：《中国全科医学》2006年第9期706-707,711,共3页Chinese General Practice

基　　金：国家自然科学基金资助项目(30300204)

摘　　要：目的探讨东北汉族人群WNK4基因多态性与原发性高血压的关系。方法259例原发性高血压患者和235例健康正常对照人群血样应用PCR-RFLP方法检测A589S多态,结果经测序验证,同时检测所有人群的血糖、血脂等生化指标。结果(1)两组研究对象基因型频率间差别无显著性意义(P>0.05),但等位基因频率间差别有显著性意义(P<0.05);(2)GT和TT基因型与收缩压及舒张压升高密切相关(P=0.041,0.032)。结论WNK4基因A589S多态可能与高血压密切相关。Objective To elucidate the association of WNK4 gene A589S polymorphism with essential hypertension in north - eastern han isolated population. Methods Genotyping was performed using polymerase chain reaction - restriction fragment length polymorphism （PCR- RFLP） and confirmed by direct sequencing. 259 unrelated patients with essential hypertension and 235 unrelated normal controls were recruited. All the subjects had routine laboratory tests. Results （ 1 ） The frequency of WNK4 genotype GG, GT and TT were 0. 63, 0. 33, 0. 04 in controls respectively, and 0. 53, 0. 43, 0. 04 in hypertensive respectively. There was no significant differences in genotypes between hypertensive group and normal controls. （ P 〉 0. 05 ）. The frequencies of G and T were 0. 80, 0. 20 in normal controls and 0. 74, 0. 26 in hypertensives respectively. And the frequencies of T allele in hypertension group is significantly higher than that in controls （25.9% versus 20. 2%, P 〈 0. 05 ）. （2） The GT and TT genotypes were closely associated with raised systolic and diastolic blood pressure （ P =0. 041,0. 032）. Conclusion The WNK4 gene A589S polymorphism is associated with essential hypertension in north -eastern han population.

关 键 词：WNK4基因 多态性 高血压 

分 类 号：R544.11[医药卫生—心血管疾病]