钙黏蛋白-18mRNA在难治性癫癎患者脑组织中的表达  

Expression of Cadherin-18 mRNA in Intractable Epilepsy

作　　者：龚云[1] 王学峰[1] 李劲梅[1] 刘凤英[1] 汪建华[1] 席志芹[1] 朱曦[1] 奕国明[2] 王玉平[3] 张国君[3] 李云林[2] 张建国[4] 

机构地区：[1]重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室,400016 [2]卫生部北京医院神外科,100730 [3]首都医科大学宣武医院,100050 [4]首都医科大学天坛医院神经外科,100053

出　　处：《中国临床神经科学》2006年第3期225-229,共5页Chinese Journal of Clinical Neurosciences

基　　金：卫生部科学研究基金资助(98-1-354);重庆市卫生局科研基金资助(01-2-031)

摘　　要：目的:研究难治性癫患者脑组织内钙黏蛋白-18(cadherin-18)mRNA的表达,拟从另一角度加深对人类难治性癫的认识。方法:在基因芯片扫描的基础上,运用RT-PCR检测钙黏蛋白-18mRNA在难治性癫患者脑部的变化。结果:基因芯片扫描显示钙黏蛋白-18mRNA在难治性癫患者脑部比正常人表达上调5.320倍,RT-PCR检测同样显示钙黏蛋白-18mRNA在难治性癫患者脑部表达增强,其电泳条带灰度值为25.547,而正常组仅为11.176。结论:结果表明特异性表达于中枢神经系统的钙黏蛋白-18mRNA在难治性癫患者脑部表达增强,提示钙黏蛋白-18可能参与了人类难治性癫的形成。Aim： To study the change of cadherin-18 mRNA expression in the brain of intractable epileptic patients so as to explore the possible mechanisms of the pathogenesis of intractable epilepsy. Methods： The microarray scanning and reverse transcription polymerase chain reaction （RT-PCR） were used to evaluate cadherin-18 mRNA expression in the brain of 40 intractable epileptic patients and 8 normal control human. Results： As compared with the normal control group, there appeared a significant increase in cadherin-18 mRNA expression in intractable epileptic patients, with microarray scanning cy5/cy3 ratio being 5.320, RT- PCR gray density of electrophoresis band being 25.547 in intractable epileptic patient group, 11.176 in normal control group, respectively. Conclusion： It is cadherin-18 mRNA, not N-cadherin or any other cadherin mRNA found to have different expression in intractable epilepsy of human. Cadherin-18 may play an important role in the pathogenesis of intractable epilepsy of human.

关 键 词：难治性癫痫 钙黏蛋白-18 基因芯片 

分 类 号：R742.1[医药卫生—神经病学与精神病学]