上皮性卵巢癌组织中seprase mRNA的表达  

Expression of seprase mRNA in epithelial ovarian carcinoma tissue

作　　者：张梦真[1] 乔玉环[1] 索振河[2] Jahn M.Nesland 

机构地区：[1]郑州大学第一附属医院妇产科,郑州450052 [2]河南省高校临床医学重点开放实验室,郑州450052 [3]挪威奥斯陆大学肿瘤医院病理科N0310奥斯陆挪威

出　　处：《郑州大学学报（医学版）》2006年第3期451-454,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：挪威奥斯陆大学访问学者基金资助

摘　　要：目的:应用FITC cRNA探针原位杂交技术检测卵巢上皮性癌组织中seprasemRNA的表达。方法:设计5′端含有T7RNA聚合酶启动子序列的PCR引物,以质粒PA15His的cDNA为模板扩增得到DNA片段,并在T7RNA聚合酶作用下,采用体外转录方法合成FITC标记的Seprase正义和反义RNA探针,对86例卵巢上皮性癌石蜡包埋组织进行原位杂交,检测seprasemRNA的原位表达,12例正常卵巢组织作对照。结果:86例卵巢癌组织se prasemRNA的表达阳性率为96.5%(83/86),显著高于在正常卵巢组织的16.16%(2/12)(P<0.01)。seprase mRNA的表达与卵巢癌组织学分级和临床分期有关,而与患者年龄、组织学类型无关。结论:在卵巢癌的发生发展中seprasemRNA起一定作用。Aim ： To explore the expression of seprase mRNA in epithelial ovarian carcinoma tissue. Methods ：PCR was performed with gene-specific primer pairs, one containing T7 promoter sequence at the 5′ end to prepare template DNA for RNA transcription. Sense and anti-sense FITC-labeled RNA probes were generated by in vitro transcription. Afterwards, 86 cases of epithelial ovarian carcinoma tissue were investigated for mRNA expression By in situ hybridization with above probes. Results： Of 86 patients with epithelial ovarian cancer, 96.5% （83/86） were positively stained for seprase mRNA. However, 16.66% （2/12） positive stainings were observed in control group, seprase mRNA expression was associated with histological grade and FIGO stage but not with histological type and age. Conclusion： Seprase mRNA is involved in the development of ovarian cancer and may be a potential predictive marker.

关 键 词：卵巢肿瘤 原位杂交 SEPRASE 

分 类 号：R737.31[医药卫生—肿瘤]