胃腺癌组织中肿瘤坏死因子相关凋亡诱导配体受体-4的表达  

Expression of tumor necrosis factor related apoptosis inducing ligand receptor-4 in gastric adenocarcinoma tissue

作　　者：僧靖静[1] 张云汉[2] 高冬玲[2] 

机构地区：[1]郑州大学第一附属医院普外科,郑州4500522 [2]河南省肿瘤病理重点实验室

出　　处：《郑州大学学报（医学版）》2006年第3期543-545,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨肿瘤坏死因子相关凋亡诱导配体4(TRAIL R4)在诱导胃癌细胞凋亡过程中的作用。方法:应用Westernblot、RT PCR、原位杂交和免疫组织化学技术,检测31例正常胃黏膜和胃腺癌组织中TRAIL R4蛋白和mRNA的表达。结果:Westernblot结果显示,正常胃黏膜组织中TRAIL R4蛋白的阳性表达率80.65%,高于其在胃腺癌组织中的阳性表达率6.45%(P<0.05);免疫组织化学结果显示,正常胃黏膜组织中TRAIL R4蛋白均有强染色,在胃腺癌组织中染色均为阴性(P<0.05);RT PCR结果显示,正常胃黏膜组织中TRAIL R4mRNA表达高于胃腺癌组织中的表达(P<0.05);原位杂交结果显示,正常胃黏膜组织中TRAIL R4mRNA染色均为阳性,而在胃腺癌组织中阳性表达率为3.23%(P<0.05)。结论:TRAIL R4在胃癌组织中的表达缺失是TRAIL特异性诱导肿瘤细胞凋亡的原因之一。Aim： To investigate the mechanism of tumor necrosis factor related apoptosis inducing ligand receptor-4 （TRAIL-R4） in the apoptosis of gastric carcinoma cell. Methods： The expression of TRAIL-R4 in 31 cases of normal gastric mueosa and 31 cases of gastric adenoeareinoma tissue was detected using PT-PCR, Wester-blot, in situ hybridization, and immunohistoehemistry. Results： The positive rate of TRAIL-R4 protein in normal mueosa（80.65% ） was significantly higher than that in gastric adenoeareinoma（6.45% ） by Western blot（ P 〈 0.05 ）. The expression of TRAIL-R4 protein was not examined in the gastric adenoeareinoma but expressed highly in normal mueosa （ 100% ）by using the technology of immunohistoehemistry SP （P 〈 0.05）. The expression of TRAIL-R4 mRNA level in normal mueosa was significantly higher than that in gastric adenoeareinoma by RT-PCR（ P 〈 0.05 ）. The results of TRAIL-R4 mRNA by using in situ hybridization showed that the positive rates were 100% and 3.23% in normal gastric mueosa and gastric adenoeareinoma tissue, respectively （ P 〈 0.05 ）. Conclusion ： The deficiency expression of TRAIL-R4 in gastric carcinoma might involve in the apoptosis of tumor cells induced by TRAIL.

关 键 词：胃腺癌 肿瘤坏死因子相关凋亡诱导配体-4 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]