神经再生素促海马神经元生长的基因芯片研究  

Changes in Gene Expressions Induced by Nerve Regeneration Factor in Cultured Hippocampal Neurons Detected with DNA Microarray

作　　者：陈益人[1] 汤欣[1] 张琦[1] 丁斐[1] 顾晓松[1] 

机构地区：[1]南通大学江苏省神经再生重点实验室,江苏南通226001

出　　处：《神经损伤与功能重建》2006年第2期84-87,共4页Neural Injury and Functional Reconstruction

基　　金：973国家自然科学基金项目(2003CB515306)

摘　　要：目的:探索中药“神经再生素”对海马神经元生长的作用及生物学机制。方法:采用海马神经元培养及基因芯片技术,中药组加入神经再生素1μg/ ml ,对照组加入等量基础培养液。培养6 h后,观察海马神经元突起长度,并提取2组的神经元总RNA,经反转录分别用荧光标记成cDNA探针,与大鼠全基因组芯片杂交,芯片扫描,后行GCOS软件处理。结果:中药组细胞突起长度(18μm)明显长于对照组(12μm)(P<0 .01)。在基因芯片15866条基因中,248条基因上调,316条基因下调,这些基因有细胞组份、生物过程生长代谢调控因子、信号转导分子、凋亡调控因子、免疫蛋白、酶等。结论:中药“神经再生素”可促海马神经元生长。差异表达的基因为寻找药物作用的基因靶点提供了基础。Objective： To study mechanisms ot growth-promoting effects of Chinese herb ＂Nerve regeneration factor＂ （NRF） on hippocampal neurons. Methods： Rat embryonic （17d） hippocampal neurons were cultured in the presence or absence of NRF （1ug/ml）. Neurite length was measured and the total RNAs were extracted and purified from control and NRF-treated neurons. The fluorescent cDNA probes were prepared through reverse transcription and hybridized with DNA microarry chips. The chips were then scanned and analyzed. Results： The averaged neuritic length of neurons treated with NRF in the NRF-treated neurons was significantly longer than that in the control （P（0.01）. Out of total 15866 genes examined, 249 were significantly up regulated and 316 were down regulated in NRF treated neurons. These differentially expressed genes induced by NRF include G-protein coupled receptors, signal transduction molecules, cell growth and metabolism regulating factors, cellular structure molecules, enzymes, immuno-globulins, and apoptosis signaling molecules. Conclusion： NRF promotes neurite growth and regulates gene expression of a broad range of important signaling molecules. DNA microarray analysis provides a sensitive approach for preliminary searching of the targets of NRF effects.

关 键 词：中药 神经再生素 海马神经元 基因芯片 

分 类 号：R741[医药卫生—神经病学与精神病学] R338[医药卫生—临床医学]