导入外源总DNA获得大豆早熟新品系  

Early Maturing Lines of Obtained through Foreign Total DNA Introduction in Soybean

作　　者：雷勃钧[1] 卢翠华[1] 钱华[1] 李希臣[1] 周思君[1] 吕云波[1] 赵铠 刘广阳[2] 杨兴勇[2] 谢纬武[3] 王斌[3] 

机构地区：[1]黑龙江省农业科学院生物技术研究中心,黑龙江哈尔滨150086 [2]黑龙江省农业科学院克山小麦所,大豆室黑龙江克山161606 [3]中国科学院遗传研究所,北京100101

出　　处：《作物学报》1996年第2期173-177,共5页Acta Agronomica Sinica

基　　金：国家自然科学基金

摘　　要：本文报道了在大豆自花授粉后,利用其形成的花粉管通道,将提取的含有早熟血缘供体大豆绥农8号的总DNA,直接导入受体大豆黑农26号中。在后代D_2代中获得3个早熟株系,熟期比受体提早15天,并迅速稳定。经异地鉴定,D90-1072品系产量比对照品种提高19.1％,于1993年进入省区试。经过对该组合的受体,供体及转化后代进行RAPD[Random Amplified Polymorphic DNA]分析,证明供体DNA片段进入受体引起后代基因组的变异。This study reports that foreign DNA was introduced into recipient Heinong 26 directly through pollen tube after self-pollination of soybean. The donor was Suinong 8 containing early maturing consanguinity. Three early maturing plant lines were obtained in D2 generation and they matured 15 days earlier than that of the recipient. The lines stabilized fast. According to the experiment results in different locations the yeild of line D90-1072 was 19.9% higher than CK and was assigned into provincial local trial in 1993. Through analysis of RAPD (Random Amplified Polymorphic DNA) to the recipient, donor and offspring of the combination , it was proved that fragments of donor DNA have gone into the genome of the recipient and caused variation in the offsprings.

关 键 词：大豆 外源DNA 早熟品种 

分 类 号：S565.102[农业科学—作物学]