反相高效液相色谱法制备纯辣椒素的研究  

Preparation of pure capsaicin with RP-HPLC method

作　　者：吴波[1] 谭文界[2] 付玉梅[3] 

机构地区：[1]广州医学院药物研究中心,广州510182 [2]湖北中医学院植化教研室,武汉430061 [3]广州医学院荔湾医院,广州510070

出　　处：《中南药学》2006年第3期178-179,共2页Central South Pharmacy

基　　金：广东省中医药管理局科研基金资助项目(1050075)

摘　　要：目的从辣椒中制备纯辣椒素。方法从辣椒中提取、分离和纯化出辣椒辣素,然后利用RP-HPLC制备纯辣椒素,并进行HPLC和MS分析。结果从辣椒中分离到一种白色片状结晶,其MS谱显示有2个分子离子峰(m/e305和m/e307);HPLC分析出现2个色谱峰,进一步用HPLC分离该结晶制得的组分其HPLC和MS均显示为单一化合物。结论根据最终化合物的MS谱与标准辣椒素质谱对比,用此方法制得的辣椒素是单体。OBJECTIVE To obtain pure capsaicin from the fruit of capsicum. METHODS Capsaicinoid was ex tracted, isolated and purified from the fruit of capsicum. Capsaicin was isolated by RP-HPLC, and analyzed by GCMS and HPLC. RESULTS A white crystal was isolated from the fruit of capsicum. Its mass spectrum indicated two molecular ion peaks at m/e 305 and m/e 307. Mass spectrum and HPLC spectrum of the sample showed pure compound. CONCLUSIONS The pure compound was identified as capsaicin according to the GC-MS data.

关 键 词：辣椒素 制备 分析 

分 类 号：R927[医药卫生—药学]