灵孢多糖对脂多糖诱导的原代多巴胺能神经元变性的保护作用  

Ganoderma Lucidum Polysaccharides Protects Rat Primary Dopaminergic Neurons Induced by Lipopolysaccharides

作　　者：杨海华[1] 徐评议[1] 刘焯霖[1] 朱雯[1] 李毅[1] 叶钦勇[1] 

机构地区：[1]中山大学附属第一医院神经内科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2006年第3期241-245,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金资助项目(30370509;30570645);留学回国人员基金资助项目(2003-406)

摘　　要：目的观察灵孢多糖对脂多糖诱导的原代多巴胺能神经元变性的保护作用。方法建立原代中脑多巴胺能神经元与胶质细胞的共培养,随机分为6组:对照组、脂多糖组(20ng/mL);单纯灵孢多糖组、脂多糖+灵孢多糖(50,100,300!O/mL)3组。通过免疫组化检测酪氨酸羟化酶,OX-42的阳性细胞数,以RT-PCR检测TNF-αmRNA和iNOSmRNA表达。结果灵孢多糖(100,300!O/mL)组的酪氨酸羟化酶阳性细胞数明显多于脂多糖组,分别为(30.1±3.1,34.2±3.2,23.4±2.8)。脂多糖组激活的小胶质细胞体积增大,细胞数量增多(30.6±4.5),TNF-αmRNA和iNOSmRNA表达增高,但经较大剂量灵孢多糖(100,300!O/mL)预处理后,小胶质细胞的激活被抑制,总的细胞数量显著减少(26.4±5.1,25.1±4.6),TNF-αmRNA和iNOSmRNA表达量降低(P<0.01)。结论灵孢多糖预处理对脂多糖诱导的原代中脑多巴胺能神经元变性具有保护作用,可能是由于灵孢多糖的预处理阻断了由脂多糖诱导的小胶质细胞的激活,从而减少了TNF-αmRNA和iNOSmRNA的表达。[Objective] To investigate the effects of Ganoderma lucidum polysaccharides （GLPS） on the protection of rat primary dopaminergic neurons induced by lipopolysaccharides （LPS）. [Methods] We co-cultured the rat primary neuron-glia. The co-cultured ceils were divided into 6 groups （n=5）： control group, LPS group（20 ng/mL）, GLPS group and LPS＋GLPS （50,100,300 p,9/mL）group. The positive cells of TH, ox-42 and the expression of TNF-ct mRNA and iNOS mRNA were observed by immunohistochemistry and RT-PCR. [ Results] The numbers of TH positive neurons in the groups of GLPS（100 p,g/mL,300 p,g/mL） were more than those in LPS group（30.1±3.1,34.2±3.2,23.4±2.8）. The microglia can be activated by LPS and the total number of microglia was increased（30.6±4.5）. The expression of TNF-α mRNA and iNOS mRNA was increased. Pretreatment with GLPS（100 μg/mL, 300μg/mL） could block LPS-induced activated microglia and the total number of microglia was also decreased （26.4±5.1,25.1±4.6）. Consequently the expression of TNF-α mRNA and iNOS mRNA was also significantly inhibited （P〈0.01）. [Conclusion] Pretreatment with GLPS could protect the rat primary DA neurons induced by LPS. The effects may be contributed to GLPS-blocked activity of microglia induced by LPS and reduced the expression of TNF-α mRNA and iNOS mRNA.

关 键 词：多巴胺能神经元 脂多糖 灵孢多糖 肿瘤坏死因子Α 一氧化氮合酶 

分 类 号：R363[医药卫生—病理学]