胃癌多基因甲基化状态分析  

Detection of multiple gene methylation in gastric cancer

作　　者：赵成海[1] 张宁[1] 卜献民[2] 李岩[3] 张海鹏[1] 

机构地区：[1]中国医科大学病理生理教研室,辽宁省沈阳市110001 [2]中国医科大学附属二院普通外科,辽宁省沈阳市110004 [3]中国医科大学附属二院消化内科,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2006年第10期1004-1007,共4页World Chinese Journal of Digestology

摘　　要：目的:检测胃癌组织中p16,hMLH1,E-cadherin和RUNX3基因甲基化状态,探讨多基因甲基化在胃癌发病中的作用.方法:采用饱和氯化钠法提取肿瘤组织、瘤旁组织及正常胃黏膜组织DNA.采用甲基化特异PCR对上述基因甲基化状态进行分析.结果:在正常胃黏膜组织中,38.9%存在E-cadherin甲基化,16.7%存在RUNX3甲基化,没有发现p16和hMLH1甲基化;在癌旁组织中,p16、hMLH1、E-cadherin和RUNX3甲基化频率分别为8.3%,4.2%,54.2%和29.2%;在胃癌组织中,上述基因甲基化频率分别为33.3%,20.8%,0.8%和54.2%.66.7%胃癌被检出存在2个或2个以上基因甲基化,明显高于癌旁组织(37.5%,χ2=4.09,P<0.05)和正常胃黏膜组织(5.6%,χ2=15.94,P<0.01),而癌旁组织则高于正常胃黏膜组织(χ2=4.16,P<0.05).有5例胃癌没有检出任何一种基因甲基化.结论:多基因甲基化是部分胃癌发展过程中一种早期事件,提示多基因甲基化在这部分胃癌的发病中起重要作用.AIM： To determine the methylation status of p16, hMLH1, E-cadherin and RUNX3 genes in gastric cancer. METHODS： DNA in gastric cancer, canceradjacent tissues and normal gastric mucosa was extracted by saturated sodium chloride （NaCl） solution. The methylation status of p16, hMLH1, E-cadherin and RUNX3 genes were detected by methylation-specific polymerase chain reaction （MSP）. RESULTS： Methylations of E-cadherin and RUNX3 were found in 38.9% and 16.7% normal gastric mucosa, respectively. Methylations of p16 and hMLH1 were not observed in any normal gastric mucosa. The frequencies of p16, hMLH1, E-cadherin and RUNX3 methylations were 8.3%, 4.2%, 54.2% and 29.2%, respectively,in cancer-adjacent tissues, and 33.3%, 20.8%, 70.8% and 54.2%, respectively, in gastric cancer. Methylations of two or more genes were found in 66.7% gastric cancer, which was significantly higher than that in cancer-adjacent tissues （37.5%, %2 = 4.09, P 〈 0.05） and normal gastric mucosa （5.6%, %2 = 15.94, P 〈 0.01）. Methylations were also significantly different between cancer-adjacent tissues and normal gastric mucosa （%2 = 4.16, P 〈 0.05）. None of the above gene mythylation was detected in 5 gastric cancer tissues. CONCLUSION： Multiple gene methylation is an early event in some gastric cancers and it plays an important role in the development of these gastric cancers.

关 键 词：胃癌 甲基化 P16 HMLHL E—cadherin RUNX3 

分 类 号：R735.2[医药卫生—肿瘤]