整合素连接激酶反义寡脱氧核苷酸对喉鳞状细胞癌Hep-2细胞株的作用  

Study on the effects of antisense oligodeoxynucleotide of integrin-linked kinase on Hep-2 cell lines in larynx squamous cell carcinoma.

作　　者：蹇华 陈乾美[2] 耿勇 裴华[4] 

机构地区：[1]贵州省第二人民医院耳鼻咽喉科,贵阳550004 [2]贵阳医学院耳鼻咽喉科 [3]解放军第四十四医院耳鼻咽喉科 [4]贵阳医学院基础免疫学教研室

出　　处：《贵州医药》2006年第5期387-390,共4页Guizhou Medical Journal

摘　　要：目的探讨整合素连接激酶(ILK)反义寡脱氧核苷酸(ASODN)对喉鳞状细胞癌 Hep-2细胞株生物学特性的影响及抑制ILK活性以控制喉鳞状细胞癌细胞生长的可能性。方法应用脂质体包裹的ILK ASODN转染喉鳞状细胞癌Hep-2细胞,阻断ILK表达;应用光学显微镜观察凋亡细胞的形态学改变;通过细胞生长曲线检测细胞的增殖活性;于处理后120h采用膜联蛋白(Annexin- V)／碘化丙啶(PI)联合标记法于荧光显微镜下观察细胞凋亡;于处理后72h以流式细胞术测定细胞凋亡及细胞周期。结果 ASODN组转染后,Hep-2细胞生长受到抑制,细胞凋亡增加,细胞周期发生显著变化:G0／G1期细胞数增多,S期及G2／M期细胞数则减少,与对照组相比,差异有显著意义 (P<0．05);而SODN及NODN组与对照组相比则无明显变化(P>0．05)。结论 ILK ASODN可以抑制喉鳞状细胞癌Hep-2细胞的生长和增殖,诱导细胞凋亡;应用反义技术抑制ILK活性可能对喉鳞状细胞癌的治疗有意义。Objective To investigate the influence of antisense oligodeoxynucleotide（ASODN） of integrin-linked kinase（iLK） on the eytobiologieal features of Hep-2 cell lines in larynx squamous cell carcinoma, and explore the possibility to control the growth of larynx eareinoma cells by inhibiting ILK aetivity. Methods Hep-2 cells were transfeeted using lipofeetine-indueed ASODN of ILK, and the expression of ILK was bloeked. The morphological change was observed by light mieroscopy while the inhibition of growth was studied by cell growth curves method. Cell apoptosis was detected by Annexin-V/PI labeling by fluorescence mieroscopy 120h after treatment, and eell eyele phases and apoptosis were analyzed by using flow eytometry 72h after treatment. Results The cell growth was inhibited effeetively by ASODN （P〈0. 05）. Compared with the control group, the ASODN group experienced inerease in apoptosis of Hep-2 ceils （P〈0. 01）, and eell eyele phases were altered signifieantly： G0/ G1 phase cells were inereased （P〈0. 05）. Conclusion ASODN of ILK ean signifieantly inhibit cell growth and proliferation, and induce cell apoptosis of Hep-2 lines in larynx squamous eell eareinoma, suggesting it may be possible to control larynx eareinoma by inhibiting ILK aetivity through antisense teehnology.

关 键 词：反义寡脱氧核苷酸 整合素连接激酶 喉鳞状细胞癌细胞株 细胞凋亡 细胞周期 

分 类 号：R739.65[医药卫生—肿瘤]