托吡酯对海人酸致癫痫状态大鼠海马caspase-3表达的影响  

Effect of topiramate on caspase-3 expression in rat hippocampus following kanic acid induced status epilepticus

作　　者：孙建英[1] 汤树海[2] 迟兆富[1] 吴伟[1] 刘学伍[1] 

机构地区：[1]山东大学齐鲁医院神经内科,山东济南250012 [2]山东大学齐鲁医院麻醉科,山东济南250012

出　　处：《山东大学学报（医学版）》2006年第5期463-466,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金立项资助课题(Y2001C10)

摘　　要：目的：观察海人酸（KA）诱导的癫痫持续状态（SE）大鼠海马区caspase-3表达的变化及托吡酯（TPM）对其的影响。方法：用TPM预处理；用KA诱导成年雄性Wistar大鼠SE模型。分别于SE终止后第3、12、24、48h取脑，行HE染色做海马普通病理检查，并用免疫组化方法检测caspase-3的表达变化。结果：嗜酸性神经元在SE后24～48h最多；caspase-3的表达亦于SE后同一时点明显增加；TPM在SE后24和48h点明显降低caspase-3的表达。结论：在实验性SE模型中，TPM可降低海马caspase-3的表达，从而减轻SE后脑损伤。Objective： To observe the effect of topiramate （TPM） on the caspase-3 expression in rat hippocampus following kanic acid（KA）induced status epilepticus （SE）, Methods： SE was induced in adult male Wistar rats following gastric injection TPM （ 18 mg/kg·d^-1 ） and abdominal injection of KA（10 mg/kg）, and 3, 12, 24 and 48 hours later, HE staining and immunohistochemical staining were used to examine the caspase-3 expression. Resuits： HE staining showed that acidophily neurons were the most in the period of 24 - 48 hours after SE, and caspase-3 expression increased significantly in the same period but declined at 24-and 48 hour-time points by TPM. Conclusion： In experimental SE model, the caspase-3 expression increases 24 hours after SE but decreases by TPM, and therefore the brain damage is reduced.

关 键 词：托吡酯 癫痫持续状态 半胱氨酸天冬氨酸蛋白酶 

分 类 号：R742.1[医药卫生—神经病学与精神病学]