肉桂GC特征指纹图谱方法学研究  

Methodological Study on GC Fingerprint of Volatile Oil from Cinnamomum cassia Presl

作　　者：方琴[1] 魏刚[2] 丁平[1] 徐鸿华[1] 

机构地区：[1]广州中医药大学,广州510405 [2]广州中医药大学第一附属医院实验中心,广州510405

出　　处：《中药新药与临床药理》2006年第3期188-192,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省"十五"中药现代化重大项目"建立广东道地药材GAP示范基地"子课题(KB012015)

摘　　要：目的建立肉桂挥发油GC分析色谱条件,初步拟定肉桂挥发油特征指纹图谱指标成分群。方法采用GC法对广东、广西不同产地以及基地栽培肉桂的挥发油进行主要成分比较分析,从中归纳出11个主要共有峰。色谱条件:HP-5石英毛细管色谱柱(CrosslinkedMehylsiloxame,30m×0.321mm×0.25μm);柱温:100℃,以20℃.min-1升至140℃,再以2℃.min-1升至200℃;进样口温度240℃;检测器(FID)温度300℃;流速为0.4mL.min-1;分流比为50∶1。结果以11个主要共有峰为评价指标,GC色谱条件的精密度、稳定性、重现性良好。结论GC法建立肉桂挥发油指纹图谱,方法准确可靠,专属性强,特征明显,可用作肉桂挥发油质量控制和鉴别的依据。Objective To establish the method of fingerprint analysis for volatile oil from Cinnamomum cassia Presl and to determine the main characteristic components. Methods The main components of the volatile oil of Cinnamomum cassia Presl from different habitats of Guangdong and Guangxi provinces and from GAP base were compared by GC fingerprinting, and 11 common components were determined. The chromatogram conditions were as follows： the GC system consisted of Flame Ionization Detector （FID） and HP6890 gas chromatograph with a HP- 5 column （Crosslinked Mehyl siloxame, 30 m × 0. 321 mm × 0.25 μm）, the temperatures of sample vent and FID were 240 ℃ and 300 ℃ respectively and the column programmed temperature was elevated from 100 ℃ to 140 ℃ at the rate of 20 ℃ · min^- 1 and then from 140 ℃ to 200 ℃ at the rate of 2 ℃ · min^-1, the carrier gas was N2 and its flow rate was 0.4 mL · min^-1, and the split ratio was 50 ： 1. Results With 11 components as indexes, the RSD of precision, reproducibility and stability of GC fingerprinting method is in the range of 5 % . Conclusion A good fingerprint of Cinnamomum cassia Presl has been established. The method is reliable, accurate and can be applied for the quality control of Cinnamomum cassia Presl.

关 键 词：肉桂 挥发油 指纹图谱 GC 方法学 质量控制 

分 类 号：R284.1[医药卫生—中药学]