环氧化酶-2在人、大鼠乳腺癌及人乳腺癌细胞株的表达及其意义  

The significance of expressions of cyclooxgenase-2 in human or rat breast cancer and breast cancer lines in Vitro

作　　者：郭贵龙[1] 李卓英[2] 周素梅[1] 金纯[1] 林包荣[1] 张筱骅[1] 

机构地区：[1]温州医学院第一附属医院肿瘤外科,325000 [2]温州医学院第一附属医院重症监护病房,325000

出　　处：《肿瘤研究与临床》2006年第5期292-295,共4页Cancer Research and Clinic

基　　金：温州医学院科研发展基金资助项目(2003×01)

摘　　要：目的探讨环氧化酶-2(COX-2)在人、大鼠乳腺癌及人乳腺癌细胞株的表达及其意义。方法应用免疫组织化学、Westernbloting、RT-PCR法检测人乳腺癌组织及配对的癌旁组织、大鼠致癌组、选择性COX-2抑制剂尼美舒利(NIM)组、人乳腺癌细胞株中COX-2的表达,MMT法检测细胞株的生长率。结果正常人、大鼠乳腺组织不表达COX-2mRNA、蛋白,癌旁组织表达明显增高,肿瘤组织COX-2mRNA、蛋白显著增高,NIM明显下调大鼠乳腺癌COX-2的表达;MDA-MB-231细胞COX-2呈强表达,MCF-7细胞不表达COX-2;NIM显著抑制MCF-7、MDA-MB-231细胞的增生。结论COX-2可作为预防乳腺癌的分子靶点;NIM对MDA-MB-231细胞的抑制更为有效,提示COX-2选择性抑制剂对ER(-)的乳腺癌有更好的预防作用,COX-2的分子靶向治疗将成为乳腺癌预防和治疗的新途径。Objective To investigate the significance of expressions of cyclooxygenase-2 （COX-2） in human or rat breast cancers and human breast cancer lines in vitro. Methods Immunohistochemistry, RTPCR, Western blot assays were used to assess the expressions of COX-2 in cancerous tissues, paired tissues adjacent to breast cancer, normal tissues far from neoplasms, including human, rat breast cancers, and two kinds of＇human breast cancer lines. Results Strong expressions of COX-2 mRNA and protein were detected in human or rat breast cancers, and increased expression in paired tissues adjacent to cancers, but no expressions in normal tissues. There was high expression of COX-2 in MDA-MB-231 breast cancer cell line, no expression in MCF-7 cell line. Conclusions COX-2 could serve as a target of breast cancer. Selective COX-2 inhibitors may be more useful in the chemoprevention and therapy of ER negative breast cancer, and COX-2 would be a new molecular target for breast cancer treatment in the future.

关 键 词：环氧化酶-2 乳腺癌 人乳腺癌细胞株 

分 类 号：R737.9[医药卫生—肿瘤]