高效液相色谱法测定大豆中的维生素E含量及其与粗脂肪含量的线性回归分析  

THE DETERMINATION OF VITAMIN E USING HPLC AND THE LINEAR REGRESSION OF THE CONTENTS OF VITAMIN E AND OIL IN SOYBEAN

作　　者：王丽[1] 宋志峰[1] 纪锋[1] 金卫东[1] 黄璜[1] 于志晶[2] 唐晓博[3] 

机构地区：[1]吉林省农业科学院大豆研究中心,公主岭136100 [2]吉林农业大学研究生院,长春130118 [3]吉林市农业科学院,长春132101

出　　处：《大豆科学》2006年第2期113-117,共5页Soybean Science

基　　金：美国伊利诺斯州瑞利生公司资助项目

摘　　要：利用高效液相色谱法测定1000份大豆资源中的维生素E含量，使用荧光检测器检测（Ex=295nm，Em=330nm），以α-β-γ-δ-生育酚为标准品，加入母育酚为内标，计算大豆中维生素E各并构体的含量及总含量，α-β-γ-δ-生育酚的加标回收率分别为101．10％、97．50％、96．94％和96．30％，测得野生大豆资源中维生素E含量范围0．05～．34mg／g，栽培资源0．12～0．38mg／g。同时按国标方法测定粗脂肪含量，其中野生资源粗脂肪含量范围3．01％～20．60％，栽培资源12．00％～23．59％。通过线性回归分析，明确了大豆中维生素E与粗脂肪含量呈正相关的线性关系。The contents of the vitamin E in 1000 soybean occessions were determined by high performance liquid chromatography with fluorescence detection （Ex= 295nm, Em=330nm）. The isomers of vitamin E were determined by the standards α-β-γ-δ-tocopherols and the internal standard of the tocol. The vitamin E contents was 0.05-0.34g/mg in wild soybean resources, 0.10-0. 38g/mg in cultivated soybean resources, respectively. The average recoveries of α-β-γ-δ-tocopherol were 101.10%, 97.50%, 96.94% and 96.30%, respectively. Meanwhile, the oil contents of soybean resources were determined by national standard method （GB 2906-82）. Their range were 3.01%-20.60% in wild soybean resources,8.00%-23.59% in cultivated soybean resources, respectively. The positive correlation between the contents of vitamin E and oil was confirmed by the analysis of linear regression.

关 键 词：高效液相色谱法 大豆 维生素E 粗脂肪 线性回归分析 

分 类 号：S565.1[农业科学—作物学]