榄香烯对多药耐药人红白血病细胞株的影响  

Effect of β-elemene on multi drug resistance in K562/ADM cells

作　　者：赵瑾瑶[1] 杨佩满[1] 邵淑娟[1] 

机构地区：[1]大连医科大学组织胚胎学教研室,辽宁大连116027

出　　处：《大连医科大学学报》2006年第3期184-186,共3页Journal of Dalian Medical University

摘　　要：[目的]探讨抗肿瘤药β-榄香烯对多药耐药细胞系的影响。[方法]利用MTT，荧光染料Hoechst33342染色、透射电镜的方法观察β-榄香烯对多药耐药性（mdr）人红白血病细胞株K562／ADM细胞的生长和凋亡的影响。[结果]β-榄香烯对K562／ADM细胞的抑制作用在5—40μg／mL的药物浓度范围内呈上升趋势，加药组24—72h范围内细胞凋亡率不断增加，浓度40μg／mL72h时达高峰62．73％±，二者呈现药物浓度和作用时间依赖性（P〈0．05或P〈0．01）。[结论]诱导细胞凋亡是β一榄香烯抗肿瘤细胞作用机制之一。[ Objective] To investigate the inhibitory mechanism of β- elemene to multi drug resistance in K562/ADM cells. [ Methods] The growth and apoptosis of K562/ADM ceils treated with β - demene was determined by MTT, hoechst 33342 staining and electron microscope（EM）. [ Results] The inhibitory affection and apoptotic rate significantly increased during treated with 5 - 40 μg/ mL for 24 -72 h of β- elemene ,the maximal inhibitory rate reached to 62.73% ± after treated 72 h（ P 〈 0.05） .β- elemene have obvious affection of suppression growth and induce apoptosis to K562/ADM cells , which was depended on dose of drug and treated time to some extent. [ Conclusion] Induced apoptosis is one of its resistance tumor mechanism of β- elemene. ap

关 键 词：细胞凋亡 Β-榄香烯 K562/ADM细胞系 多药耐药 

分 类 号：R733.7[医药卫生—肿瘤]