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作 者:黄世全[1] 黄新[1] 张大乐[1] 李锁平[1] 常胜合[2]
机构地区:[1]河南大学生命科学学院,河南开封475001 [2]郑州大学离子束生物工程实验室,河南郑州450052
出 处:《麦类作物学报》2006年第3期8-11,共4页Journal of Triticeae Crops
基 金:河南省杰出青年科学基金项目(02120000900)。
摘 要:为合理利用优良种质资源,采用酸性聚丙烯酰胺凝胶电泳(A-PAGE)对44个啤酒大麦品种进行醇溶蛋白的遗传多样性研究。结果表明,44个啤酒大麦品种共分离出33种相对迁移率不同的醇溶蛋白谱带,没有公共谱带,即参试材料在33个醇溶蛋白等位变异位点上均存在多态性。44个啤酒大麦品种共得到29种不同的醇溶蛋白图谱类型,其中24种图谱为单个材料所独有,另外5种图谱分别为几个品种所共有,说明啤酒大麦醇溶蛋白遗传多态性丰富。聚类分析结果与醇溶蛋白多态性分析结果相一致。Genetic: diversity of germplasm was important in breeding. In order to use germplasm resource more effecicntly, the genetic diversity of hordein among 44 beer barleys was analyzed by acidic poly acrylamide gel electrophoresis (A-PAGE). 33 hordein patterns were identified in those materials, and no one was uniform, which indicated that 33 alleles in these materials were polymorphic. 29 types of hordein patterns were obtained from the tested materials, which showed that the hordein patterns of beer barleys were notably various. 24 materials had their unique hordein patterns, the other 5 types of hordein patterns were common in several materials. Heterotic grouping mapping showed that 44 materials were clustered into 2 major groups, and the first group was clas,;ified into 2 subgroups. The findings suggested that the hordein patterns in beer barley were greatly warious.
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