三七总皂苷对阿尔茨海默病大鼠脑胆碱能神经的保护作用(英文)  

Protective effects of panax notoginseng saponins on cholinergic neurons in rats with Alzheimer disease

作　　者：钟振国[1] 屈泽强[1] 王乃平[1] 王进声[1] 谢智光[1] 张凤芬[1] 张雯艳[1] 卢忠朋[1] 

机构地区：[1]广西中医学院药理学教研室新药研究开发中心,广西壮族自治区南宁市530001

出　　处：《中国临床康复》2006年第19期174-176,F0003,共4页Chinese Journal of Clinical Rehabilitation

基　　金：广西科技厅资助项目(桂科回0144007);广西人事厅资助项目(人发[2002]30);广西教育厅资助项目(桂教科研[2001]401)~~

摘　　要：背景:现代医学目前对阿尔茨海默病尚无有效的治疗方法,三七总皂苷治疗阿尔茨海默病已引起重视和关注,但其作用机制仍不十分清楚。目的:观察具有化瘀止血、活血定痛功效的三七总皂苷对阿尔茨海默病动物模型大脑胆碱能神经病理损害的保护作用。设计:完全随机分组设计,对照试验。单位:广西中医学院神经科学研究所。材料:实验于2003-06/2005-04在广西中医学院中药药效研究实验室完成,选用清洁级健康Wistar大鼠90只,雌雄各半。其中15月龄老年鼠75只,3月龄青年鼠15只。方法:实验于2003-06/2005-04在广西中医学院中药药效研究实验室(广西重点实验室)完成。①选用清洁级健康Wistar大鼠90只,雌雄各半。其中15月龄老年鼠75只,3月龄青年鼠15只。设3月龄15只为青年对照组。于15月龄75只中随机抽取15只作为老年对照组。对其余60只大鼠进行造模。以D-半乳糖腹腔注射致亚急性损伤合并鹅膏蕈氨酸损毁双侧大脑Meynert基底核建立阿尔茨海默病大鼠模型。青年对照组和老年对照组均在相同条件下以生理盐水作平行对照。②2周后将存活的造模大鼠随机分为4组:模型组,三七总皂苷高、低剂量组和石杉碱甲组,每组12只。三七总皂苷高、低剂量组分别按200和100mg/kg灌胃三七总皂苷(云南玉溪维和制药厂生产),1次/d;石杉碱甲组按0.3mg/kg灌胃给药,1次/d,连续4周;模型组、青年对照组和老年对照组以等体积的生理盐水灌胃,连续4周。③给药结束后行大脑组织病理切片,利用免疫组织化学方法检测大脑切片胆碱乙酰基转移酶免疫反应活性及阳性神经元数量及形态学改变。应用IBAS图像分析系统对胆碱乙酰转移酶免疫阳性神经元进行图像分析处理,测定胆碱乙酰转移酶免疫阳性细胞平均截面积和平均吸光度(A值);用显微镜测微尺测定计算胆碱乙酰转移酶阳性神经元数目。④采用单�BACKGROUND： There are no effective methods to cure Alzheimer disease （AD）. Now, researches have shown that panax notoginseng saponins （PNS） play an important role in improving AD, but its mechanism is unclear. OBJECTIVE： To observe the protective effect of PNS characterized by removing blood stasis to stop bleeding and promoting blood circulation to relieve pain on pathological lesion of cholinergic neuron in rat with AD. DESIGN： Completely randomized grouping design and controlled study. SETTING： Neuroseience Institute of Guangxi Traditional Chinese Medical University. MATERIALS： This experiment was completed in the Chinese Herb Pharmacodynamic Laboratory of Guangxi Traditional Chinese Medical University between June 2003 and April 2005. A total of 90 health Wistar rats of clean grade and half gender were selected in this study. Among them, there were 75 old rats with 15 months old and 15 young rats with 3 months old. METHODS： This experiment was completed in the Chinese herb Pharmacodynamie Laboratory （Key Laboratory） of Guangxi Traditional Chinese Medical University between June 2003 and April 2005. ① A total of 90 healthy Wistar rats of clean grade and half gender were selected in this study. Among them, there were 75 old rats with 15 months old and 15 young rats with 3 months old. Fifteen young rats with 3 months old were regarded as young control group, and other 15 selected from 75 rats with 15 months old were regarded as old control group, The rest 60 rats were modeled on the basis of subacute injury induced by intravenous injection of D-galactose and bilateral cerebral Meynert basal nuclei injured by ibotenic acid. Parallel control was performed with saline on rats in young control group and old control group under the same condition. ② Two weeks later, survival modeling rats were divided randomly into 4 groups： model group, high-dosage PNS group, low-dosage PNS group and huperzine A group with 12 in each group. Rats in high-and low-dosage PNS groups were perfused

关 键 词：三七皂甙 阿尔茨海默病/中药疗法 疾病模型 动物 胆碱O-乙酰转移酶 

分 类 号：R749.611[医药卫生—神经病学与精神病学]