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作 者:姚琴[1] 丛玲[1] 汪越胜[1] 陈明洁[1] 杨广笑[1] 何光源[1]
机构地区:[1]华中科技大学中英HUST-RRes作物遗传工程和基因组学联合实验室,武汉430074
出 处:《遗传》2006年第6期695-698,共4页Hereditas(Beijing)
基 金:国家973科技基金项目(编号:2002CB111302);国家自然科学基金(编号:30370807)~~
摘 要:通过基因枪介导转化无载体框架序列的基因最小框架是一种新的基因转化技术。在应用此新转化系统获得转1Ax1小麦植株的基础上,着重研究了转基因植株中外源1Ax1基因表达框的遗传。结果表明:无载体框架结构的1Ax1基因在T1代中呈现3∶1的分离,符合单基因位点孟德尔遗传模式。首次在小麦中揭示了无载体框架序列基因遗传转化技术的可行性,并且证实了外源基因最小表达框在转基因后代中的稳定遗传。Transformation with gene minimal expression cassettes without vector backbone sequence is a new transformation technique. In the current research, the inheritance of the foreign 1Ax1 gene cassettes were analyzed in transgenic plants recovered with gene cassettes lacking vector backbone sequences. The results showed that linear 1Ax1 gene cassettes were stably expressed and separated at the rate of 3 to 1 in the seeds of T, generation. It suggested the functional copies of 1Ax1 gene cassettes were integrated in one sites, abiding by Mendelian genetic law. It revealed firstly the feasibility of the transformation technique with gene cassettes, and proved the inheritance of transgene cassettes in subsequent generations.
关 键 词:无载体框架结构 转基因小麦 1Ax1基因 高分子量麦谷蛋白亚基
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