外源谷胱甘肽(GSH)对水鳖Zn^(2+)毒害的缓解作用  

Alleviatory Effect of Exogenous Glutathione(GSH) on Hydrocharis dubia Toxicated by Zn^(2+)

作　　者：赵娟[1] 施国新[1] 徐勤松[1] 王学[1] 许丙军[1] 胡金朝[1] 

机构地区：[1]南京师范大学生命科学学院,南京210097

出　　处：《热带亚热带植物学报》2006年第3期213-217,共5页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金(30370083);江苏省教育厅自然科学基金(03KJBI80062);江苏省重点实验室开放基金项目资助

摘　　要：研究在10 mg L-1 Zn2+毒害下外施10-50 mg L-1梯度浓度的还原型谷胱甘肽(GSH)对水鳖(Hydrocharis dubia)的保护酶(SOD、CAT、POD)活性、GSH、可溶性蛋白质、叶绿素、H2O2含量以及O2.-产生速率的影响。结果表明,相对单一的Zn2+毒害,施用外源GSH可明显减轻毒害症状,植物体内GSH含量增加了10.71%-35.71%,O2.-的产生速率最低降至78.2%,H2O2含量最低降至62.7%。植物体内可溶性蛋白含量和CAT、SOD、POD的活性最大分别增加了74.2%、108.2%、61.4%、19.5%。随GSH浓度增大,缓解能力下降,在培养液中最佳缓解浓度为20-40 mg L-1。The effect of 10 mg L^-1 Zn^2＋ together with various concentrations of glutathione （GSH） on the changes in activity of protective enzymes （ SOD, CAT, POD）, generation rate of O2^·-, and the contents of GSH, soluble protein, chlorophyll and H2O2 in leaves of Hydrocharis dubia were determined. The results showed that the addition of endogenous GSH in treatments obviously increased the content of GSH in leaves by 10.71% to 35.71%, generation rate of O2^·- was lowered to 78.2% and H2O2 was scavenged to 62.7% as compared to the treatment with 10 mg L^-1 Zn^2＋ alone without GSH. The content of soluble protein increased at most by 74.2%, and the activities of CAT, SOD, POD increased at most by 108.2%, 61.4%, 19.5%, respectively. It is obvious that GSH relieve the toxic of Zn^2＋ to some extent. The most effective concentrations of GSH against Zn^2＋ stress are 20- 40 mg L^-1 in culture solution.

关 键 词：还原型谷胱甘肽 ZN^2+ 水鳖 缓解作用 

分 类 号：X173[环境科学与工程—环境科学]