Screening of Anti-endotoxin Components from Radix Isatidis  

作　　者：王文清 方建国 刘云海 谢委 

机构地区：[1]Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2006年第2期261-264,共4页华中科技大学学报（医学英德文版）

基　　金：This project was supported by a grant from the NationalNatural Science Foundation ( Nos .39800193 ,39870872 and39170877) and the Foundation of Ministry of Health of Chi-na (No .98-2-110)

摘　　要：To find a component with the strongest anti-endotoxin effect from the four components （F021 , F022, F023, and F024 ） of chloroform extract （F02） of Radix Isatidis, the protective effects of the five components on endotoxin-challenged mice and their effects on the production of TNFα and IL-6 by macrophages from the endotoxin-challenged mice were compared. It was found that the five components all had protective effects on endotoxin-challenged mice in terms of mortality. The mortalities of the mice challenged by 2.42 mg/kg endotoxin were 30 %, 50 %, 20 %, 50 % and 60 0% after treatment with F02, F021 , F022 , F023 , and F024. The 5 components all had inhibitory effects on the production of TNFα and IL-6 by macrophages from the mice. At a concentration of LPS of 50 ng/mL, the inhibitory rates of F02, F021 1, F022, F023, and F024 for the production of TNFα and IL-6 were 76.54 %, 30. 86 %, 78.60 %, 36. 63 %, 2.06 % and 75.85 %, 18.45 %, 77. 68 %, 24. 41 %, 3. 64% and at the dose of 100 ng/mL, the inhibitory rates were 49. 65 %, 16.86%, 66.97 %, 13. 39 %, 7.16 % and 59.78 %, 1.28 %, 61.86 %, 2.08 %, 1.44 %, respectively. It is concluded that the component F022 of Radix Isatidis has the strongest anti-endotoxin effect.To find a component with the strongest anti-endotoxin effect from the four components （F021 , F022, F023, and F024 ） of chloroform extract （F02） of Radix Isatidis, the protective effects of the five components on endotoxin-challenged mice and their effects on the production of TNFα and IL-6 by macrophages from the endotoxin-challenged mice were compared. It was found that the five components all had protective effects on endotoxin-challenged mice in terms of mortality. The mortalities of the mice challenged by 2.42 mg/kg endotoxin were 30 %, 50 %, 20 %, 50 % and 60 0% after treatment with F02, F021 , F022 , F023 , and F024. The 5 components all had inhibitory effects on the production of TNFα and IL-6 by macrophages from the mice. At a concentration of LPS of 50 ng/mL, the inhibitory rates of F02, F021 1, F022, F023, and F024 for the production of TNFα and IL-6 were 76.54 %, 30. 86 %, 78.60 %, 36. 63 %, 2.06 % and 75.85 %, 18.45 %, 77. 68 %, 24. 41 %, 3. 64% and at the dose of 100 ng/mL, the inhibitory rates were 49. 65 %, 16.86%, 66.97 %, 13. 39 %, 7.16 % and 59.78 %, 1.28 %, 61.86 %, 2.08 %, 1.44 %, respectively. It is concluded that the component F022 of Radix Isatidis has the strongest anti-endotoxin effect.

关 键 词：Radix Isatidis ENDOTOXIN ACTIVITY MORTALITY TNF-α IL-6 MICE 

分 类 号：R91[医药卫生—药学]