类胰蛋白酶对血管内皮细胞表达干细胞因子的作用及机制  

Effects of mast cell tryptase on the expression of stem cell factor in endothelial cell line and its mechanism

作　　者：陆超[1] 马永杰[1] 买买提祖农.买苏尔 赵凤娣[1] 殷莲华[1] 

机构地区：[1]复旦大学上海医学院生理与病理生理学系,上海200032 [2]新疆医科大学病理生理学教研室,乌鲁木齐830054

出　　处：《复旦学报（医学版）》2006年第3期291-294,300,共5页Fudan University Journal of Medical Sciences

基　　金：上海市卫生局科技发展基金(034087);国家自然科学基金(30470689)资助

摘　　要：目的研究类胰蛋白酶对血管内皮细胞(ECV304细胞系)表达干细胞因子的作用及其机制。方法采用RT-PCR方法检测类胰蛋白酶刺激后ECV304细胞炎症介质干细胞因子(stem cell factor,SCF)表达情况,用Western blot方法研究p38和JNK的表达及磷酸化。采用单因素方差分析干细胞因子mRNA的表达以及t检验分析p38和c-Jun N末端激酶(JNK)磷酸化。结果类胰蛋白酶能够上调ECV304细胞的干细胞因子mRNA的表达,类胰蛋白酶也可以诱导细胞内p38的磷酸化,以上作用均可被PAR2单克隆抗体抑制。结论在ECV304细胞中,类胰蛋白酶经蛋白酶激活受体2通过p38的磷酸化,最终上调表达干细胞因子。Purpose To study the effects and mechanism of mast cell tryptase on endothelial cell （ECV304 cell line）. Methods The tryptase-induced expression of stem cell factor in ECV 304 cells was detected by reverse transcription-polymerase chain（RT-PCR）. The phosphorylation of p38 and JNK in ECV304 cells was analyzed by Western blotting. Data was analyzed by the One-way-ANOVA test and the student＇s test. Results Tryptase could up-regulate the expression of stem cell factor in ECV 304 cells and induce phosphorylation of p38, which could be inhibited by the anti-PAR-2 monoclonal antibody SAM11. Conclusions The results indicate that tryptase could activate p38 through PAR2 receptor and subsequently SCF expression in ECV304 cells.

关 键 词：类胰蛋白酶 血管内皮细胞 干细胞因子 蛋白酶激活受体2 

分 类 号：R363[医药卫生—病理学]