斑马鱼p53基因结构比对分析及其在生态毒理学中的应用  

Structure Alignments of Zebrafish p53 Gene and Its Application in Ecotoxicology

作　　者：顾颖[1] 李延[1] 陈蔚丰[1] 尹大强[1] 赵庆顺[2] 

机构地区：[1]南京大学环境学院污染控制与资源化国家重点实验室,南京210093 [2]南京大学模式动物研究所医药生物技术国家重点实验室,南京210061

出　　处：《生态毒理学报》2006年第1期45-51,共7页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金项目(No.20577022;20377022)

摘　　要：斑马鱼p53基因由11个外显子和10个内含子组成,外显子1和外显子11部分序列不参与编码蛋白质．其 cDNA核苷酸序列共2089bp,包含一个1122 bp的开放阅读框(Open Reading Frame,ORF)(44 bp-1165 bp),这个 ORF编码373个氨基酸．另外在3’端有924 bp是非翻译区(3’-UTR),5’端同样也存在43 bp的非翻译区(5’- UTR);12种脊椎动物p53氨基酸序列的保守性分析结果表明,在一些特殊的区域,12种氨基酸序列表现出了很高的保守性,这些区域和p53的抑癌功能密切相关．研究结果预示斑马鱼p53基因是一种理想的环境致突变物研究分子模型,在生态毒理学研究中具有良好的应用前景．Zebrafish p53 gene structure and amino acid sequence conservation of twelve vertebrates were analyzed. The results showed that zebrafish p53 gene comprised 11 exons and 10 introns. Exon 1 and part of exon 11 encode untranslated region （UTR）of mRNA. The p53 cDNA was 2089 bp long containing an open reading frame （ORF）of 1122 bp. This ORF encoded a polypeptide with 373 amino acids. The 5＇-UTR and 3＇-UTR were 43 bp and 924 bp respectively. Protein sequence alignments of p53 gene among 12 vertebrates including human showed a remarkable conservation in several domains, which had been shown to be critical for p53 function. The research indicated that zebrafish p53 gene could be used as a molecular model to evaluate the genotoxicity of environmental chemicals in the future.

关 键 词：斑马鱼 P53基因 环境致突变物 生态毒理学 

分 类 号：X171.5[环境科学与工程—环境科学]