全反式维甲酸对人胰腺癌细胞生长及Survivin基因表达的影响  

Effects of all-trans retinoic acid on growth of pancreatic cancer cells and Survivin gene expression

作　　者：骆霞岗[1] 苗毅[1] 

机构地区：[1]南京医科大学第一附属医院胰腺外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第6期389-392,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生厅135重点学科基金资助项目(苏卫科教[2001]31号)

摘　　要：目的:观察全反式维甲酸(ATRA)对人胰腺癌细胞株PC-3生长的抑制作用以及细胞株PC-3 Survivin基因表达的变化。方法:应用MTT比色法检测细胞增殖抑制率,RT-PCR法测定Survivin基因表达,流式细胞仪检测细胞凋亡。结果:经过ATRA作用后,PC-3细胞增殖受到抑制,呈时间和剂量依赖性。凋亡比例明显增加,Survivin mRNA的表达随着作用时间的延长而呈逐渐下降趋势。结论:ATRA对人胰腺癌细胞株PC-3的生长有抑制作用,同时可诱导其凋亡,提示其可能与Survivin基因表达下调有关。Objective： To observe the inhibitiory effects of all-trans retinoie acid （ATRA） on the pancreatic cancer cells growth in vitro and Survivin gene expression induced by ATRA, Methods： The inhibition rates of proliferation were detected by MTT assay. RT-PCR and flow cytometry were used to detect the expression of Survivin gene and the apoptotic rates. Results： After the treatment with ATRA, the proliferation of PC-3 was inhibited in dose-dependent and time-dependent manner,and the apoptotic rates of PC-3 cells were increased significantly. The expression of Survivin mRNA was decreased obviously in PC-3 cells receiving ATRA treatment compared with those receiving no treatment. Conclusion： ATRA can inhibit pancreatic cancer cells growth,induce cells apoptosis. Survivin gene probably contributes to the apoptosis of pancreatic cancer cells induced by ATRA.

关 键 词：全反式维甲酸 胰腺癌细胞 细胞凋亡 SURVIVIN 

分 类 号：R735.9[医药卫生—肿瘤]