神经干细胞联合胶质细胞源性神经营养因子脑内移植治疗帕金森病的实验研究  

Experimental study of intracerebral co-graft of neural stem cells and glial cell line-derived neurotrophic factor for treating Parkinson′s disease

作　　者：王磊[1] 曾水林[1] 朱建宝[1] 李涛[1] 

机构地区：[1]东南大学基础医学院解剖教研室神经生物研究所,江苏南京210009

出　　处：《南京医科大学学报（自然科学版）》2006年第6期424-427,F0002,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省科技厅自然科学基金资助项目(BK2002056)

摘　　要：目的:探讨胶质细胞源性神经营养因子(glial cell line-derived neurotrophic factor,GDNF)对脑内移植神经干细胞(neural stem cells,NSCs)的存活、分化及对帕金森病(Parkinsons’disease,PD)模型大鼠的治疗作用。方法:采用原代培养胚胎14.5天的胎鼠腹侧中脑NSCs,单独或经GDNF预处理后移植入PD模型大鼠的纹状体,观察NSCs在纹状体的分化情况,以及PD模型大鼠旋转行为的变化。结果:联合GDNF移植NSCs比单纯移植的NSCs较多的转化为多巴胺能神经元,并能够改善PD模型大鼠的旋转行为。结论:GDNF可以促进中脑来源的NSCs向多巴胺能神经元转化,并对PD模型大鼠有较好的治疗作用。Objective： To study the effects of intracerebral co-graft of neural stem cells （NSCs） and glial cell line-derived neurotrophic factor （GDNF） on Parkinson＇s disease （PD）. Methods： NSCs were isolated from embryonic （E14.5 d） rats and cultured by using serum-free medium. Then NSCs were grafted into the striatums of PD rats with GDNF. Tyrosine Hydroxylase （TH）-positive cells were gradually increased at 30 d after transplantation in the striatum of PD rats. Results： Compared with NSCs group, more TH- positive cells were found in the NSCs＋GDNF group transplanted into the striatums of PD rats. And NSCs＋GDNF group could alleviate the apomorphine （APO）-induced rotations of PD rats. Conclusion： GDNF may promote the NSCs derived from E14.5 rat embryonic mesencephlon to differentiate into dopaminergic nenrons and has the obvious therapeutical effects on PD rats.

关 键 词：神经干细胞 帕金森病大鼠 胶质细胞源性神经营养因子 多巴胺能神经元 

分 类 号：R742.5[医药卫生—神经病学与精神病学]