端粒酶逆转录酶与Bcl-2在口腔黏膜癌变过程中的表达及意义  

Expression of human telomerase reverse transcriptase and Bcl-2 in premalignant and malignant lesions of human oral mucosa

作　　者：吴国英[1] 冯振卿[2] 彭韬[2] 

机构地区：[1]南京医科大学口腔医学院口腔内科,江苏南京210029 [2]南京医科大学病理学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第6期450-452,F0003,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教育厅自然科学研究基金资助项目(03KJD320148)

摘　　要：目的:研究口腔黏膜癌前病变癌变过程中人端粒酶逆转录酶(hTERT)和Bcl-2的表达。方法:采用原位杂交和免疫组化方法对口腔黏膜单纯增生(HP)9例,轻度异常增生(LD)11例,中度异常增生(MD)10例,重度异常增生(原位癌CIS)9例,鳞癌(SCC)11例,进行hTERTmRNA及Bcl-2蛋白的检测。结果:hTERT和Bcl-2在口腔黏膜癌前病变和鳞癌中均有异常表达,CIS及SCC组织中hTERT的表达明显高于HP、LD和MD(P<0.05);SCC中Bcl-2表达明显高于HP和异常增生组织(P<0.05)。结论:端粒酶的活化和Bcl-2表达的增强在口腔黏膜癌前病变和鳞癌的发生和发展中起着重要作用,它们的异常表达有助于口腔黏膜癌前病变和鳞癌的早期诊断。Objective： To study the expression of human telomerase reverse transcriptase（hTERT） and Bcl-2 in premalignant and malignant lesions of human oral mucosa. Methods： HTERT mRNA in 9 cases of oral mucosal hyperplasia, 11 cases of light dysplasia, 10 cases of medium dysplasia, 9 cases of carcinomas in situ and 11 cases of squamous cell carcinomas was detected by in situ hybridization, and Bcl-2 was detected by immunohistochemical. Results： HTERT and Bcl-2 were abnormally expressed in premalignant and malignant lesions of human oral mueosa. There was a significant elevation of hTERT in CIS and SCC （P 〈 0.05）. There was a significant elevation of Bcl-2 in SCC（P 〈 0.05）. Conclusion： Telomerase activity and Bcl-2 expression play an important role in genesis and development of oral precancerous lesions and squamous cell carcinoma. The examination of hTERT and Bel-2 expression can help early diagnosis of oral malignant lesions.

关 键 词：癌前病变 鳞癌 人端粒酶逆转录酶 BCL-2蛋白 口腔黏膜癌 

分 类 号：R781.59[医药卫生—口腔医学]